miR-101对乳腺癌患者的诊断及对乳腺癌细胞自噬和凋亡的影响

doi:10.3969/j.issn.1006-6233.2023.08.02

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Abstract Objective: To investigate the expression and clinical characteristics of serum miR-101 in patients with breast cancer and its effects on autophagy and apoptosis of MCF-7 cells. Methods: A total of 96 patients with breast cancer treated from June 2021 to October 2022 were selected as the observation group, 41 patients with benign breast hyperplasia were selected as the benign group, and 35 healthy subjects were selected as the normal control group. The expression level of miR-101 in serum of the 3 groups was detected by qRT-PCR. The expression levels of serum miRNA-101 in different clinicopathological features of breast cancer patients were compared. The diagnostic value of miR-101 was analyzed by ROC curve. Breast cancer cell line (MCF-7) was transfected with miR-101 mimic reagent. The expression of miR-101 in MCF-7 and normal breast cell line (HBL-100) was detected by qPCR. The cell proliferation was detected by CCK-8 method. The expressions of autophagy and apoptosis genes and proteins were detected by qPCR and Western blot. The apoptosis rate of each group was detected by flow cytometry. Results: The relative expression of miRNA-145 in observation group (n= 96,0.27) was significantly lower than that in benign group (n= 41,0.63) and control group (n= 35,1.00), the difference was statistically significant (P<0.01). The expression of serum miRNA-101 was significantly different in lymph node metastasis, differentiation degree, tumor diameter and TNM stage (P<0.01). ROC curve analysis showed that the area under the curve was 0.723(95%CI: 0.813-0.924) and 0.875(95%CI: 0.835-0.981) in the three groups of comparison, respectively. The results of CCK-8, qPCR and Western blot showed that over-expression of miR-101 inhibited the proliferation of MCF-7 cells, significantly increased the gene and protein expression of BAX in MCF-7 cells (P<0.01), and decreased the gene and protein expression of BCL-2, LC3B and Beclin-1 in McF-7 cells (P<0.01). The apoptosis rate of MCF-7 cells was increased. Conclusion: In patients with breast cancer, the expression level of serum miR-101 is significantly decreased, which may be a potential diagnostic indicator of breast cancer. In addition, miR-101 overexpression inhibited the proliferation of MCF-7 breast cancer cells, inhibited autophagy, and promoted apoptosis. These results provide important clues for further research on the mechanism of miR-101 in the occurrence and development of breast cancer, and provide new strategies for the early diagnosis and treatment of breast cancer.

Key words： Breast cancer Autophagy Apoptosis

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	Articles by authors
	SONG Yaqi
	ZHANG Zhisheng
	KONG Lingxia
	et al

Cite this article:

SONG Yaqi,ZHANG Zhisheng,KONG Lingxia, et al. The Effect of miR-101 on the Diagnosis of Breast Cancer Patients and Autophagy and Apoptosis of Breast Cancer Cells[J]. HeBei Med, 2023, 29(8): 1240-1245.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.08.02 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I8/1240

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