慢性阻塞性肺病合并细菌感染患者CD4<sup>+</sup>CD25<sup>+</sup>T调节细胞及相关细胞因子表达水平及意义

doi:10.3969/j.issn.1006-6233.2023.06.013

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Abstract Objective: To study the expression levels and clinical significance of CD4⁺CD25⁺T regulatory cell and related cytokines of interleukin-4 (IL-4), IL-12 and interferon-γ (IFN-γ) in patients with chronic obstructive pulmonary disease (COPD) complicated with bacterial infection. Methods: A total of 108 patients with COPD admitted between August 2020 and August 2022 were included as the research subjects, and they were divided into infected group (n=53) and control group (n=55) according to whether the patients were complicated with bacterial infection. The expression levels of CD4⁺CD25⁺ regulatory T cell and IL-4, IL-12 and IFN-γ were detected in the peripheral blood samples, and the differences between the two groups were compared, and the relationship between levels of related indicators and COPD with infection was analyzed. The 28-d prognosis of patients was statistically analyzed, and the predictive value of the above indicators on prognosis was analyzed. Results: The levels of serum IL-4 and IL-12 of COPD patients in infected group were higher than those in control group while the IFN-γ/IL-4 was lower than that in control group (P<0.05). Follow-up results showed that 15 patients died within 28 d, with a mortality rate of 13.89%. The level of serum IL-4 of COPD patients in death group was higher than that in control group while the levels of CD4⁺CD25⁺, IL-12, IFN-γ and IFN-γ/IL-4 were lower than those in control group (P<0.05). Logistics regression analysis showed that serum levels of IL-4 and IL-12 were associated with COPD with pulmonary infection (P<0.05), and CD4⁺CD25⁺, IL-12 and IFN-γ/IL-4 were associated with the prognosis of COPD patients (P<0.05). ROC curve showed that the AUC values of CD4⁺CD25⁺, IL-12, IFN-γ/IL-4 and the combination of the three were 0.747, 0.827, 0.709, and 0.924 respectively in judging prognosis of COPD patients. Conclusion: CD4⁺CD25⁺ Treg cell and related cytokines are related to COPD infection and disease progression. Detection of CD4⁺CD25⁺ Treg distribution as well as IL-4, IL-12 and IFN-γ can provide reference information for diagnosis of pulmonary infection and evaluation of prognosis in patients with COPD.

Key words： Chronic obstructive pulmonary disease Regulatory T lymphocytes Interleukin-4 Interferon-γ

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	XING Zhenquan
	ZHAO Guangqiang
	LI Liuxia

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XING Zhenquan,ZHAO Guangqiang,LI Liuxia. Expression Levels and Significance of CD4⁺CD25⁺T Regulatory Cell and Related Cytokines in Patients with COPD Complicated with Bacterial Infection[J]. HeBei Med, 2023, 29(6): 947-951.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.06.013 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I6/947

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