槲皮素对口腔鳞癌细胞增殖凋亡等细胞生物学行为及p38/JNK MAPK信号通路的影响

doi:10.3969/j.issn.1006-6233.2023.04.02

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Abstract Objective: To investigate the effect of quercetin on the proliferation, apoptosis, migration, invasion and p38/JNK MAPK signaling pathway of oral squamous cell carcinoma cells. Methods: Oral squamous cell carcinoma Tac8113 cells cultured in vitro were used as research subjects, and blank control group, low-dose quercetin group (25μmol/L), high-dose quercetin group (50μmol/L) and positive control group (cisplatin 4μg/mL) were set respectively. The effect of quercetin on the proliferation of oral squamous cell carcinoma Tac8113 cells was detected by CCK-8 method; the effect of quercetin on the apoptosis of oral squamous cell carcinoma Tac8113 cells was detected by flow cytometry; the migration and invasion of oral squamous cell carcinoma Tac8113 cells was detected by Transwell assay; WB method was used to detect the expression of p38/JNK MAPK signaling pathway-related proteins. Results: Compared with the blank control group, the proliferation activity, migration number and invasion number of Tac8113 cells in the low-dose and high-dose quercetin groups and the positive control group decreased, and the apoptosis rate increased (P<0.05). Compared with Tac8113 cells in the high-dose quercetin group and the positive control group, the proliferation activity, the number of migration and the number of invasion decreased, and the apoptosis rate increased (P<0.05), in a dose-dependent manner; There was no significant difference in the proliferation activity, migration number, invasion number and apoptosis rate of Tac8113 cells between the high-dose quercetin group and the positive control group. (P>0.05). Compared with the blank control group, the expressions of p-p38, p-JNK and Caspase-3 in the low-dose and high-dose quercetin groups were increased (P<0.05); compared with the low-dose quercetin group, the protein expressions of p-p38, p-JNK and Caspase-3 in the high-dose quercetin group were increased (P<0.05), in a dose-dependent manner; The differences in p38 and JNK protein expression among the blank control group, quercetin low dose group, quercetin high dose group, and positive control group were not significant (P>0.05). Conclusion: Quercetin can reduce the proliferation activity, migration and invasion number of oral squamous cell carcinoma Tac8113 cells, and induce Tac8113 cell apoptosis, which may be achieved by activating the p38/JNK MAPK signaling pathway.

Key words： Quercetin Oral squamous cell carcinoma cells Cell biological behavior p38/JNK MAPK signaling pathway

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	Articles by authors
	HU Shuohong
	ZHENG Xuebin
	LI Fujie

Cite this article:

HU Shuohong,ZHENG Xuebin,LI Fujie. Effects of Quercetin on Cell Biological Behaviors Including Proliferation Apoptosis and p38/JNK MAPK Signaling Pathway in Oral Squamous Carcinoma Cells[J]. HeBei Med, 2023, 29(4): 535-540.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.04.02 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I4/535

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