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Ras and ERK1/2 Protein Expression in Cancer Tissues of Patients with Type 2 Diabetes Mellitus and Colorectal Cancer: Significance and Correlation with Lymph Node Metastasis and Survival |
NIU Shu, DONG Lina, et al |
The People's Hospital of Shijiazhuang, Hebei Shijiazhuang 050011, China |
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Abstract Objective: To investigate the expression levels of Ras-ERK1/2 signaling in patients with type 2 diabetes mellitus (T2DM) and colorectal cancer (CRC), analyze their relationship with lymph node metastasis, and explore their correlation with survival status. Methods: A total of 59 patients who underwent colorectal cancer surgery at People's Hospital of Shijiazhuang from March 2019 to January 2021 were selected. Patients were divided into the diabetes group (n=29) and non-diabetes group (n=30) based on the presence of concurrent diabetes. Immunohistochemical methods were used to detect the expression levels of Ras and ERK1/2 proteins in surgically resected cancer tissues. Differences in lymph node metastasis were analyzed using the Log-rank test. All patients were followed up for 36 months, and survival conditions were statistically compared. The Spearman correlation analysis was used to analyze the correlation between Ras and ERK1/2 expression and survival period. Results: Ras and ERK1/2 were expressed in both CRC and CRC combined with T2DM, mainly concentrated in the cytoplasm of cancer cells. Compared with the non-diabetes group, the positive expression rates of Ras and ERK1/2 in the CRC combined with T2DM group were significantly increased (P<0.05). The lymph node metastasis rate in the CRC combined with T2DM group was significantly higher than that in the pure CRC group (P<0.05). Compared with the Ras and ERK1/2 negative expression group, the lymph node metastasis rate was significantly higher in the Ras and ERK1/2 positive expression group (P<0.05). The Spearman correlation analysis showed that the protein expression of Ras and ERK1/2 in colorectal cancer patients was negatively correlated with median survival time (P<0.05). Conclusion: Positive expression of Ras-ERK1/2 proteins is positively correlated with lymph node metastasis and negatively correlated with the survival period of colorectal cancer patients, making it an important target for early diagnosis and prognosis assessment.
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