LncRNA MAFG-AS1调节miR-331-3p/SERPINE1轴对胃癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2023.11.04

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Abstract Objective: To investigate the impact of the long non-coding RNA (LncRNA) recombinant human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G antisense RNA1 (MAFG-AS1) on the malignant biological behavior of gastric cancer cells by regulating the miR-331-3p/SERPINE1 axis. Methods: QRT-PCR was applied to detect the expression of MAFG-AS1,miR-331-3p,and SERPINE1 in gastric cancer tissues (n=30),paracancerous tissues (n=30),human gastric mucosa normal epithelial cells GES-1,human gastric cancer cells BGC-823,SGC-7901,and MGC-803; BGC-823 cells were randomly grouped into ctrl group,pcDNA3.1 group,pcLncRNA MAFG-AS1 group,pcLncRNA MAFG-AS1+miR-NC group,and pcLncRNA MAFG-AS1+miR-331-3p group; qRT-PCR was applied to detect the levels of MAGG-AS1,miR-331-3p,and SERPINE1 in BGC-823 cells in each group; CCK-8 method was applied to detect the activity of BGC-823 cells in each group; EDU method was applied to detect the proliferation of BGC-823 cells in each group; Transwell was applied to detect the migration and invasion of BGC-823 cells in each group; Western blot was applied to detect the expression of SERPINE1,PCNA,and MMP-2 in BGC-823 cells in each group; Double Luciferase reporter gene experiment was applied to verify the relationship between MAFG-AS1 and miR-331-3p,miR-331-3p and SERPINE1. Results: In comparison to adjacent tissues,gastric cancer tissues exhibited significantly increased expressions of MAFG-AS1 and SERPINE1 mRNA (3.01±0.37 vs 1.02±0.12 and 2.49±0.26 vs1.01±0.10; t=28.022 and 29.000; P<0.05) and markedly decreased miR-331-3p expression (0.32±0.03 vs 1.03±0.07; t=51.063; P<0.05).Furthermore,various gastric cancer cells displayed notably higher MAFG-AS1 and SERPINE1 mRNA expressions and lower miR-331-3p expression in comparison to GES-1 cells (P<0.05).Among the gastric cancer cell lines,BGC-823 cells exhibited the most significant changes,so we selected BGC-823 cells for subsequent experiments.Compared to the control group and pcDNA3.1 group,the pcLncRNA MAFG-AS1 group showed significantly higher levels of MAFG-AS1,SERPINE1 mRNA,cell viability (A value),EDU-positive cell rate,numbers of migration and invasion cells,as well as SERPINE1,PCNA,and MMP-2 protein levels,while miR-331-3p mRNA levels were significantly lower (P<0.05).In comparison to the pcLncRNA MAFG-AS1 group and the pcLncRNA MAFG-AS1+miR-NC group,the pcLncRNA MAFG-AS1+miR-331-3p group showed significantly lower SERPINE1 mRNA levels,cell viability (A value),EDU-positive cell rate,numbers of migration and invasion cells,as well as SERPINE1,PCNA,and MMP-2 protein levels,whereas miR-331-3p mRNA levels were significantly higher (P<0.05).MAFG-AS1 negatively regulated miR-331-3p expression,and miR-331-3p negatively regulated SERPINE1 expression. Conclusion: Overexpression of LncRNA MAFG-AS1 may upregulate SERPINE1 expression by negatively regulating miR-331-3p,thereby promoting proliferation,migration and invasion of BGC-823 cells.

Key words： LncRNA MAFG-AS1 miR-331-3p SERPINE1 Gastric cancer cells

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	SONG Yutao
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SONG Yutao,WANG Feng,LI Fan, et al. Impact of LncRNA MAFG-AS1 on the Malignant Biological Behavior of Gastric Cancer Cells by Regulating the miR-331-3p/SERPINE1 Axis[J]. HeBei Med, 2023, 29(11): 1777-1783.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.11.04 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I11/1777

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