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| Expression and Clinical Significance of miR-29b-3p and FOXO3A in Colon Cancer Tissues |
| LI Bowen, HAN Yuansheng, HUANG Gaojun, et al |
| Dangyang People's Hospital, Hubei Dangyang 444100, China |
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Abstract Objective: To discuss the clinical significance of microRNA-29b-3p (miR-29b-3p) and forkhead box O3A (FOXO3A) in colon cancer tissues by detecting their expression level. Methods: A total of 82 patients with colon cancer treated in our hospital from January 2015 to January 2017 were selected. Colon cancer tissues and adjacent normal tissues ≥5cm from the tumor margin were collected during the operation. Real-time fluorescence quantification (qRT-PCR) was used to detect the expression levels of miR-29b-3p and FOXO3A mRNA in various tissues, at the same time, Western blot was used to detect the expression level of FOXO3A protein in each tissue. Pearson method was used to analyze the correlation between miR-29b-3p and FOXO3A mRNA expression in colon cancer; Kaplan-Meier method was used to analyze the relationship between the expression of miR-29b-3p, FOXO3A mRNA and the survival rate of patients; the factors affecting the 5-year mortality of patients with colon cancer were analyzed by COX regression. Results: The expression level of miR-29b-3p in colon cancer tissues (1.55±0.40) was higher than that in adjacent normal tissues (1.01±0.23). The expression levels of FOXO3A mRNA and protein in colon cancer tissues [(0.48±0.13), (0.72±0.19)] were lower than those in adjacent normal tissues [(0.99±0.25), (1.43±0.37)]. The difference was statistically significant (t=23.87, 35.501, 33.318, P<0.05). The expressions of miR-29b-3p and FOXO3A mRNA in colon cancer were correlated with TNM stage and lymph node metastasis of colon cancer patient (P<0.05). There was a negative correlation between the expression of miR-29b-3p and FOXO3A mRNA in colon cancer (r=-0.479, P<0.05). The 5-year overall survival rate of patients with low expression of miR-29b-3p (83.33%) was higher than that of patients with high expression (55.00%), and the difference was statistically significant (χ2=6.820, P<0.05). The 5-year overall survival rate of patients in the FOXO3A mRNA high-expression group (88.64%) was higher than that of patients in the low-expression group (47.37%), and the difference was statistically significant (χ2=16.005, P<0.05). The high expression of miR-29b-3p and low expression of FOXO3A mRNA in TNM Ⅲ stage and colon cancer tissues were risk factors for 5-year death in colon cancer patients (P<0.05). Conclusion: The expression of miR-29b-3p is high, and the expression of FOXO3A mRNA and its protein is low in colon cancer tissues. miR-29b-3p and FOXO3A mRNA are correlated with TNM stage, lymph node metastasis, and prognosis.
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2023, Vol. 29 
