SIRT1在M/CMCS治疗慢性牙周炎牙槽骨丢失的机制研究

doi:10.3969/j.issn.1006-6233.2023.10.004

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Abstract Objective: To investigate the mechanism of SIRT1 in the treatment of alveolar bone loss and TLR4/NF-κB signaling in rats with chronic periodontitis by metronidazole carboxymethyl chitosan gel. Methods: Fifty rats were randomly divided into five groups: NC group, Model group, M/CMCS group, MNZ group and M/CMCS+EX527 group, with 10 rats in each group. Clinical indicators of periodontal tissues (BI, PD) were measured in the rats. ELISA was used to measure the levels of IL-6, IL-1βand TNF-α in serum. Micro-CT was used to measure the bone mineral density (BMD), bone volume/total volume (BV/TV), and the distance between the cemento-enamel junction (CEJ) and alveolar bone crest (ABC). HE staining was performed to examine the histopathological changes in periodontal tissues. Protein immunoblotting was used to detect the expression of TLR4, NF-κB p65, and SIRT1 proteins in periodontal tissues. Results: Compared to the NC group, the rats in the model group showed significant increases in PD (2.28±0.21), BI (3.76±0.30), levels of TNF-α (234.05±11.62), IL-1β (203.62±15.31), and IL-6 (181.35±11.27) in serum, CEJ-ABC distance (615.34±51.45), and protein expression of TLR4 (0.53±0.06) and NF-κB p65 (0.75±0.08) in periodontal tissues, as well as decreases in BMD (0.32±0.03), BV/TV (31.02±1.57), and protein expression of SIRT1 (0.21±0.03) in periodontal tissues (P<0.0001). Compared to the model group, the M/CMCS group and MNZ group showed significant decreases in PD (0.81±0.08), (1.23±0.13), BI (1.24±0.12), (1.89±0.15), levels of TNF-α (75.81±7.54), (115.36±8.23), IL-1β (93.21±8.15), (122.48±9.02), and IL-6 (76.81±6.32), (105.04±7.11) in serum, CEJ-ABC distance (276.17±27.28), (361.35±30.94), and protein expression of TLR4 (0.21±0.03), (0.28±0.03) and NF-κB p65 (0.36±0.04), (0.42±0.05) in periodontal tissues, as well as increases in BMD (0.54±0.05), (0.42±0.05), BV/TV (45.91±2.06), (39.87±2.41), and protein expression of SIRT1 (0.72±0.08), (0.25±0.06) in periodontal tissues, with the M/CMCS group showing the most significant changes (P<0.0001). Compared to the M/CMCS group, the rats in the M/CMCS+EX527 group showed significant increases in BI (3.18±0.27), PD (2.10±0.18), levels of TNF-α (208.54±10.81), IL-1β (191.25±12.37), and IL-6 (173.26±10.04) in serum, CEJ-ABC distance (601.82±50.29), and protein expression of TLR4 (0.50±0.05) and NF-κB p65 (0.71±0.07) in periodontal tissues, as well as decreases in BMD (0.35±0.04), BV/TV (35.62±1.64), and protein expression of SIRT1 (0.25±0.03) in periodontal tissues (P<0.0001). Conclusion: Metronidazole carboxymethyl chitosan gel can promote alveolar bone reconstruction and repair periodontal tissues in rats with chronic periodontitis and inhibit TLR4/NF-κB signaling pathway by a mechanism related to the activation of SIRT1 expression.

Key words： Metronidazole carboxymethyl chitosan gel/M/CMCS Chronic periodontitis Alveolar bone loss TLR4/NF-κB signaling pathway SIRT1

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	YIN Haoyun
	CAO Yang
	ZHANG Ting
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YIN Haoyun,CAO Yang,ZHANG Ting, et al. Mechanisms of SIRT1 in M/CMCS for Alveolar Bone Loss and TLR4/NF-κB Signaling in Chronic Periodontitis[J]. HeBei Med, 2023, 29(10): 1606-1612.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.10.004 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I10/1606

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