阿托品对形觉剥夺性近视模型大鼠的改善作用及作用机制

doi:10.3969/j.issn.1006-6233.2023.01.014

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Abstract Objective: To investigate the improvement effect of atropine (ATR) on form deprivation myopia model rats by regulating Wnt/β-catenin (β-catenin) signaling pathway and related mechanisms. Methods: Sixty SD rats were randomly grouped into control (NC) group, form deprivation myopia model (Model) group, ATR group (3mg/kg), TAK-715 group (10mg/kg Wnt/β- catenin pathway activator TAK-715), and ATR+TAK-715 group (3mg/kg ATR+10mg/kg TAK-715), with 12 per group. The right eye of NC group and Model group was subconjunctively injected with equal amount of normal saline. After 4 weeks, the axial length and diopter of the right eye of the rats in each group were recorded; the morphology of sclera was observed by HE staining; ELISA kits were used to detect the levels of serum interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α); RT-qPCR was used to detect the mRNA expression of scleral tissue transforming growth factor β1 (TGF-β1), matrix metalloproteinase 2 (MMP-2), and matrix metalloproteinase inhibitor-2 (TIMP-2); Western blot was used to detect the expression of Wnt/β-catenin pathway proteins in sclera tissue. Results: The sclera of the rats in the NC group was normal; the sclera in the Model group became thinner, and the collagen fibers were sparsely arranged and disordered; the thickness of sclera and the diameter of collagen fibers increased in ATR group; the sclera thickness of the rats in the ATR+TAK-715 group decreased, and the fibers were arranged disorderly. Compared with the normal sclera in the NC group, the axial length, refraction, the levels of IL-1β, IL-6 and TNF-α, the mRNA level of MMP-2, and the protein levels of Wnt and MMP-2 in the Model group were obviously increased (P<0.05), the mRNA levels of TGF-β1 and TIMP-2, and the protein levels of TGF-β1, TIMP-2, and p-β-catenin/β-catenin were obviously decreased (P<0.05); compared with the Model group, the axial length, refraction, the levels of IL-1β, IL-6 and TNF-α, the mRNA level of MMP-2, and the protein levels of Wnt and MMP-2 in the ATR group were obviously decreased (P<0.05), the mRNA levels of TGF-β1 and TIMP-2, and the protein levels of TGF-β1, TIMP-2, and p-β-catenin/β-catenin were obviously increased (P<0.05), the corresponding index of TAK-715 group showed the opposite trends (P<0.05); compared with the ATR group, the axial length, refraction, the levels of IL-1β, IL-6 and TNF-α, the mRNA level of MMP-2, and the protein levels of Wnt and MMP-2 in the ATR+TAK-715 group were obviously increased (P<0.05), the mRNA levels of TGF-β1 and TIMP-2, and the protein levels of TGF-β1, TIMP-2, and p-β-catenin/β-catenin were obviously decreased (P<0.05). Conclusion: ATR may reduce inflammatory response and inhibit extracellular matrix remodeling by regulating the Wnt/β-catenin pathway, thereby improving form deprivation myopia in rats.

Key words： Atropine Wnt/β-catenin Form deprivation myopia Inflammatory response

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	LI Xiqian
	KANG Ping
	ZHOU Yu

Cite this article:

LI Xiqian,KANG Ping,ZHOU Yu. Ameliorative Effects and Mechanism of Action of Atropine in Rats with Form Deprivation Myopia Model[J]. HeBei Med, 2023, 29(1): 76-81.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.014 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/76

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