滋膵通脉饮通过调节PI3K/Akt/mTOR信号通路减轻高糖诱导的H9c2心肌细胞损伤的实验研究

doi:10.3969/j.issn.1006-6233.2023.01.006

Abstract
Figure/Table
References (15)
Related Citation (15)

Download: PDF (1436 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the possible signaling pathways (PI3K-Akt-mTOR) involved in autophagy and apoptosis of H9c2 cardiomyocytes induced by high glucose, and to investigate the mechanism of action of Zicui Tongmai Decoction in inhibiting cardiomyocyte apoptosis. Methods: SD rats were randomly divided into blank plasma group and Zicui Tongmai Decoction group, with 10 rats in each group. The corresponding medicated plasma was prepared by gavage for 7 days,the drug concentration of Zicui Tongmai Decoction group was 4.4g/mL and the gavage volume was 10mL·kg^-1·d^-1,the blank plasma group was gavaged with the same dose of sterilized ultrapure water (the administration volume was 10mL/kg). H9c2 myocardial cells were divided into normal group, blank plasma group and drug containing plasma group, fetal bovine serum, blank plasma and drug containing plasma were added respectively. A concentration gradient of 5%, 10% and 15% was set for each group. The proliferation of H9c2 cardiomyocytes in each group was compared by CCK-8 method, the cell survival rate was calculated, and the drug containing plasma was screened. H9c2 cardiomyocytes induced by high glucose were randomly divided into three groups: model group, Zicui Tongmai Decoction medicated plasma group and engliejing group, and additionally there was normal group. Expression of LC3B in H9c2 cardiomyocytes was detected 24h after administration of IF, and the expression of PI3K Akt mTOR signal pathway protein and apoptosis related protein caspase-3 was detected by Western blot. Results: Drug containing plasma and blank plasma have no significant effect on cell proliferation at the concentration level of 10%, which is used as the intervention concentration of drug containing plasma experiment. The expression of LC3B Ⅱ/LC3BⅠ decreased significantly compared with the normal group(P<0.05),but the protein expression of PI3K, P-Akt, mTOR and Caspase-3 increased compared with the normal group(P<0.05). The expression of LC3B Ⅱ/LC3BⅠin Zicui Tongmai Decoction medicated plasma group and engliejing group increased significantly compared with the model group(P<0.05),but the protein expression of PI3K,P-Akt,mTOR and Caspase-3 in Zicui Tongmai Decoction medicated plasma group and engliejing group decreased compared with the model group(P<0.05). There was no significant difference in expression of LC3BⅡ/LC3BⅠ and protein expression of PI3K,P-Akt,mTOR and Caspase-3 between Zicui Tongmai Decoction medicated plasma group and engliejing group (P>0.05). Conclusion: Zicui Tongmai Decoction can activate autophagy and inhibit apoptosis of cardiomyocytes, which may be related to the inhibition of PI3K/p-Akt / mTOR signaling pathway.

Key words： Zicui Tongmai decoction H9c2 cardiomyocytes Autophagy Apoptosis PI3K-Akt-mTOR

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.006 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/31

[1] 尧青,李勇,陈晓,等.姜黄素对糖尿病大鼠心肌自噬、凋亡及AMPK-mTOR信号通路的影响[J].中国医院药学杂志,2022,42(10):1004-1008.
[2] 杜琎,石开虎,赵扬,等.丹参酚酸B通过抑制PI3K/Akt/mTOR通路促进自噬减轻大鼠心肌纤维化的研究.[J].现代生物医学进展,2019,19(20):3812-3817.
[3] 吴刚强,熊春红,毛叶,等.滋膵通脉饮对糖尿病心肌病大鼠心肌纤维化和转化生长因子β1/Smads3信号通路的影响[J].中药新药与临床药理,2021,32(1):29-35.
[4] 吴刚强,毛叶,温小凤,等.滋膵通脉饮对糖尿病心肌病大鼠心肌重构的影响[J].中西医结合心脑血管病杂志,2021,19(2):240-243.
[5] Wang Y,Liang B,Lau WB,et al.Restoring diabetes-induced autophagic flux arrest in ischemic/reperfused heart by ADIPOR(adiponectinreceptor)activation involves both AMPK-dependent and AMPK-independent signaling[J].Autophagy,2017,13(11):1855 -1869.
[6] Li Xuefei,Hu Xiaorong,Wang Jichun,et al.Inhibition of autophagy via activation of PI3K/ Akt/ mTOR pathway contributes to the protection of hesperidin against myocardial ischemia/ reperfusio injury[J].International Journal of Molecular Medicine,2018,42(4):1917-1924.
[7] 王晓平,张雪峰,邵明燕,等.自噬信号通路在心力衰竭中的作用以及中药干预的研究进展[J].中华中医药杂志,2019,34(9):4220-4223.
[8] Fan C,Tang X,Ye M,et al.Qi-Li-Qiang-Xin aleviates isoproterenol-induced myocardial injury by inhibiting excessive autophagy activating AKT/mTOR pathway[J].Front Pharmacol,2019,10:1-4.
[9] 饶小娟,吴毓敏,王彦.麦冬皂苷介导AMPK/Akt/mTOR通路对糖尿病大鼠心肌细胞自噬的影响[J].中国循证心血管医学杂志,2018,10(11):1362-1367.
[10] 诺明,陶慧,哈森塔娜,等.舒芬太尼对心肌缺血/再灌注大鼠心肌细胞凋亡及Bcl-2、LC3Ⅱ、Beclin-1表达的影响[J].中西医结合心脑血管病杂志,2020,18(1):50-54.
[11] 殿臣,宫丽鸿,肖福龙.稳斑汤含药血清对巨噬细胞泡沫化凋亡相关蛋白Bax、Bcl2和caspase3表达的影响[J].辽宁中医杂志,2018,45(6):1309-1312.
[12] Liu X,Tan W,Yang F,et al.Shengmai injection reduces apoptosis and enhances angiogenesis after myocardial ischaemia and reperfusion injury in rats[J].Biomed Pharmacother,2018,104(8):629-636.
[13] 田瑛,邓小敏,苏冬,等.基于网络药理学探讨增液汤治疗糖尿病的作用机制[J].广西医学,2021,43(15):1868-1872.
[14] 杨嫒萍,张磊,李炜,等.黄芪甲苷对糖尿病心肌病小鼠的治疗作用及其机制[J].山东医药,2022,62(23):19-24.
[15] 陈梓健,蒋正午,高乐,等.地龙提取物对2型糖尿病心肌病的治疗作用与机制探讨[J].局解手术学杂志,2020,29(8):616-619.