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| Effect of Uremic Toxin on Calcification of Vascular Smooth Muscle Cells |
| DUAN Shuzhong, MA Sijia, YU Wenhui, et al |
| The Affiliated Hospital of Chengde Medical University, Hebei Chengde 067000, China |
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Abstract Objective: To study the respective effects of high phosphorus or high parathyroid hormone levels on smooth muscle cell calcification. Methods: Human thoracic aortic vascular smooth muscle cells (VSMC) were cultured in vitro for 14 days and divided into control group, high phosphorus group (2.6 mmol/L), high parathyroid hormone (parathyroid hormone 1-34, PTH1-34) group a (PTH1-34 concentration 10-11 mol/L), high parathyroid hormone (parathyroid hormone 1-34, PTH1-34) group b (PTH1-34 concentration 10-10 mol/L), and observed in respect of cellular calcium salt deposition after alizarin red staining; real-time quantitative PCR and western blotting were used to detect the gene and protein expression of bone morphogenetic protein 2 (BMP-2) and Runt-related transcription factor 2 (Runx-2). The intracellular calcium content of each group was determined by applying the intracellular calcium content kit. Resulst: Calcification of vascular smooth muscle cells in the high phosphorus group was detected by alizarin red staining, and expression of calcification proteins BMP-2 and Runx-2 was significantly increased by western blotting. Calcification of vascular smooth muscle cells cultured in the high parathyroid hormone group was not detected by alizarin red staining, and BMP-2 and Runx-2 expression in vascular smooth muscle cells in the high parathyroid hormone group was not increased by real-time quantitative PCR and western blotting compared with the control group. expression did not increase compared with the control group. The intracellular calcium content of cells in the high phosphorus group was significantly higher than that of the control group, and the intracellular calcium content of cells in the high parathyroid hormone a and b groups did not differ from that of normal control cells (P>0.05). Conclusion: The calcification of VSMCs was induced merely by high phosphorus, However,PTH stimulation for 14 days failed to calcify vascular smooth muscle cells.
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2022, Vol. 28 
