黄芩苷对复发性口腔溃疡模型大鼠IL-33/ST2信号通路的影响及机制研究

doi:10.3969/j.issn.1006-6233.2022.08.06

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Abstract Objective: To investigate the therapeutic effect of baicalin on model rats with recurrent oral ulcer (ROU) and its mechanism by regulating the interleukin-33/suppression of tumorigenicity 2 (IL-33/ST2) signaling pathway. Methods: Fifty rats were injected with autologous antigens to construct ROU models and randomly divided into model group, low, medium, and high dose baicalin (25, 50, 100 mg/kg) groups, and levamisole group (17.5 mg/kg); another 10 rats injected with Freund's complete adjuvant were taken as the normal group. After the completion of intragastric administration, the number, duration and interval of ulcers of rats in each group were observed and recorded; flow cytometry was used to detect the contents of CD4⁺ and CD8⁺ T in lymphocyte subsets in the peripheral blood of rats in each group; enzyme-linked immunoassay was used to detect the levels of interferon-γ (IFN-γ), IL-17, IL-2, and tumor necrosis factor-α (TNF-α) in the serum of rats in each group; reduced glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA) test kits were used to detect the levels of GSH, SOD and MDA in the serum of rats in each group; Western blotting was used to detect the levels of IL-33, ST2, and nuclear factor-kB (NF-kB) protein in the oral mucosa tissues of rats in each group. Results: Compared with the normal group, the number of ulcers in the model group increased, the duration increased, and the interval time prolonged, the CD4⁺ and CD4⁺/CD8⁺ decreased, the CD8⁺ increased, the contents of IFN-γ, IL-17, IL-2, TNF-α, and MDA increased, the contents of GSH and SOD reduced, the expression of IL-33, ST2, and NF-kB proteins was up-regulated (P<0.05). Compared with the model group, the number and duration of ulcers in the low, medium and high dose baicalin groups decreased, and the interval time prolonged, the CD4⁺ and CD4⁺/CD8⁺ increased, the CD8⁺, the contents of IFN-γ, IL-17, IL-2, TNF-α, and MDA decreased, the contents of GSH and SOD increased, the expression of IL-33, ST2, and NF-kB proteins was down-regulated (P<0.05), and they were dose-dependent. There was no statistically significant difference in indicators between the high-dose baicalin group and levamisole group (P>0.05). Conclusion: Baicalin has a significant therapeutic effect on ROU model rats. It may inhibit the IL-33/ST2 pathway, regulate the imbalance of T lymphocyte subsets, and improve inflammatory response and oxidative damage.

Key words： Baicalin IL-33/ST2 signaling pathway Recurrent oral ulcer T lymphocyte subsets Oxidative stress

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	Articles by authors
	WEI Ya'nan
	LIU Xiaohui
	MA Yuanxin

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WEI Ya'nan,LIU Xiaohui,MA Yuanxin. Effect of Baicalin on IL-33/ST2 Signaling Pathway in Rats with Recurrent Oral Ulcers and Its Mechanism[J]. HeBei Med, 2022, 28(8): 1262-1267.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.08.06 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I8/1262

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