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| Influences of GSK343 on EZH2/STAT4/IFN-γ Pathway and the Killing Effect of γδT Cells in Glioma Cells |
| XIA Liqiong, YANG Minghua, XIE Yan |
| Mianyang Central Hospital, Sichuan Mianyang 621000, China |
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Abstract Objective: To investigate the influences of GSK343 on the enhancer of Zeste homolog 2 (EZH2)/signal transducer and activator of transcription 4 (STAT4)/interferon-γ (IFN-γ) pathway and the killing effect of γδT cells. Methods: Human glioma cell line U373 was cultured in vitro, and the control group (normal cell cultured, no drug treatment) and GSK343 group (GSK343 treatment) were set up. The proliferation of U373 cells and the killing effect of γδT cells on U373 cells were detected by CCK-8 method; the apoptosis of U373 cells was detected by flow cytometry; the cell morphology was observed by microscopy; and the expressions of EZH2, p-STAT4 and IFN-γ proteins in U373 cells were detected by Western blot. Results: With the increase of GSK343 concentration, the inhibition rate of U373 cell proliferation increased gradually, and the GSK343 half inhibition concentration (IC50) of U373 cells was in the range of 20-50 μmol/L. Therefore, 50μmol/L were selected as the best treatment concentration in the GSK343 group for subsequent experiments. Compared with the control group, the expression levels of EZH2 protein in GSK343 group decreased significantly (P<0.05), and the apoptosis rate, p-STAT4, IFN-γ protein expression level and the killing activity of γδT cells to U373 cells in each group were significantly increased (P<0.05), irregular morphology and cytoplasmic swelling U373 cells killing of γδT cells increased. Conclusion: GSK343 can inhibit the expressions of EZH2 proteins, promote the expression of STAT4 phosphorylation and IFN-γ protein, thus enhancing the killing effect of γδT cells on U373 cells, inhibit the proliferation and induce apoptosis of U373 cells.
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2022, Vol. 28 
