重组变构人TRAIL联合5-FU对裸鼠结直肠癌移植瘤的抑制作用

doi:10.3969/j.issn.1006-6233.2022.05.06

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Abstract Objective: To observe the antitumor effect of recombinant mutant human tumor necrosis factor (TNF)-related apoptosis-inducing ligand (rmhTRAIL) or known as circular permuted TRAIL (CPT) in combination with 5-fluorouracil (5-FU) on transplanted tumor of colorectal cancer in nude mice. Methods: Human colorectal cancer (CRC) cell line HCT116 was inoculated subcutaneously into the middle lateral axillary of each nude mouse to establish transplanted tumor model in nude mice. After randomization, 5-FU was injected into caudal vein and CPT was injected into abdominal cavity. The general situation of nude mice was observed, and the weight and tumor length and diameter of nude mice were measured. Finally, all the animals were sacrificed, and then the tumor tissues were stripped, photographed and weighed. The tumor cell apoptosis was determined by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)/2 4',6-diamidino-2-phenylindole (DAPI) assay, and observed and photographed by Laser confocal microscope. Results: CRC bearing nude mice model was successfully established, and the nude mice were all in good condition. CPT and 5-FU alone could inhibit the growth of transplanted tumor in nude mice, and the comparison of that with control group has statistical difference (P<0.01). The combination of the two drugs had more significant inhibitory effect on the growth of transplanted tumor in nude mice, and the high-dose CPT group was the most significant (P<0.01). In comparison with control group, the tumor volume and weight of CPT, 5-FU and co-treatment groups were significantly reduced (P<0.05). CPT, 5-FU and their combination promoted the apoptosis of tumor cells, especially in the combination group, and the difference was significant in comparison with control (P<0.05). Conclusion: CPT alone or in combination with 5-FU can significantly suppress the growth of human CRC HCT116 transplanted tumors in nude mice, and promote the apoptosis of tumor cells CRC tumor-bearing nude mice.

Key words： Transplanted tumor Colorectal cancer Tumor necrosis factor related apoptosis inducing ligand Nude mice

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.05.06 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I5/731

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