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| Effect of Sevoflurane Based on BDNF/TrkB Signaling Pathway on Dendritic Spine Development in Rat Offspring During Late Pregnancy |
| SU Juan, et al |
| The Second Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi 830000, China |
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Abstract Objective: To explore the effect of sevoflurane anesthesia on dendritic spine development in late pregnant rat offspring and its mechanism. Methods: Pregnant rats were divided into control group, sevoflurane-1h group, sevoflurane-3h group and sevoflurane-6h group, they were anesthetized with 2.3% sevoflurane respectively. P31 offspring rats were subjected to water maze test and executed with high CO2 concentration. Brain tissue was taken for electrophysiological experiments to detect long duration enhancement in CA1 region of hippocampus, Golgi staining to assess dendritic spine status and western blot to detect BDNF/TrkB protein expression.Results: Compared to the control group, the latency to evade was not altered in the water maze experiment in late pregnant rat offspring P35 pups anesthetized with sevoflurane for 1h, whereas the latency to evade increased significantly in the sevoflurane-3h and sevoflurane-6h groups, and the number of platform crossings and quadrant III dwell time decreased significantly. In addition, sevoflurane anesthesia at 3h and 6h caused a significant decrease in the slope of fEPSP and a significant decrease in the density and length of dendritic spines in the pups, however, there was no effect on the fEPSP and dendritic spine status in the sevoflurane-1h group. At the same time, the expression of PSD-95, BDNF and p-TrkB in hippocampal tissues of young rats was significantly reduced by 3h and 6h sevoflurane anesthesia.Conclusion: The reduced learning and memory capacity in late pregnant rat pups after prolonged anaesthesia is caused by reduced BDNF expression, which results from reduced p-TrkB expression mediating reduced PSD-95 protein, leading to impaired dendritic spine development.
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2022, Vol. 28 
