Per1基因对口腔鳞癌细胞增殖侵袭和迁移的影响

doi:10.3969/j.issn.1006-6233.2022.02.08

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Abstract Objective: To investigate the effect and mechanism of Per1 gene on cell proliferation, invasion and migration of oral squamous cell carcinoma by regulating the Wnt/β-catenin signaling pathway. Methods: Human oral squamous cell carcinoma cells SCC-15 were cultured, and si-Per1 and si-NC were transfected into SCC-15 cells, as si-Per1 group and si-NC group. Per1 gene expression level was detected by RT-qPCR, cell proliferation ability was detected by MTT, cell invasion and migration ability were detected by Transwell chamber, and Ki-67, CyclinD1, MMP-2, MMP-9, β-catenin protein expression were detected by Western blot test. Results: Compared with the HOK group, the expression level of Per1 gene in Cal-27, SCC-4 and SCC-15 was significantly reduced (P<0.05). Compared with adjacent tissues, the expression level of Per1 gene in oral squamous cell carcinoma tissues was significantly reduced (P<0.05). Compared with the si-NC group, the OD value of the si-Per1 group was significantly increased (P<0.05), and the expression of Ki-67 and CyclinD1 protein was significantly increased (P<0.05). Compared with the si-NC group, the invasive and migration cells in the si-Per1 group were significantly increased (P<0.05), and the protein expression of MMP-2 and MMP-9 was significantly increased (P<0.05). Compared with the si-NC group, the β-catenin protein expression in the si-Per1 group was significantly reduced (P<0.05). Conclusion: High expression of Per1 gene can inhibit the proliferation, invasion and migration of oral squamous cell carcinoma cells, which is related to the inhibition of Wnt/β-catenin signaling pathway.

Key words： Per1 Wnt/β-catenin signaling pathway Oral squamous cell carcinoma Proliferation Invasion Migration

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.02.08 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I2/220

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