miR-141对股骨头坏死骨髓间质干细胞活性及VEGF/TGF-β<sub>2</sub>蛋白的影响

doi:10.3969/j.issn.1006-6233.2022.12.003

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Abstract Objective: To investigate the effects of Mir-141 on the activity of bone marrow mesenchymal stem cells and VEGF/TGF-β₂ protein in femoral head necrosis. Methods: Patients with femoral fracture and steroid-induced femoral head necrosis admitted to our hospital were randomly divided into 4 groups after bone marrow extraction, culture and identification of MSCs during surgery,including group A (hMSCs cells from patients with femoral neck fracture), group B (hMSCs cells from patients with steroid-induced femoral head necrosis), group C (group B + Mir-141-sirNA-NC), and group D (group B + Mir-141-SiRNA). MTT was used to detect cell activity. The apoptosis rate was detected by flow cytometry. The protein and mRNA levels of VEGF and TGF-β₂ were detected by western blot and RT-PCR, respectively. Luciferase assay demonstrated the regulatory effect of Mir-141 on VEGF and TGF-β₂. Results: Compared with group A, the activity of hMSCs cells in group B was decreased at 24h, 48h and 72h (P<0. 05), while the OD values of hMSCs cells in groups B and C were insignificant at 24h, 48h and 72h (P>0. 05). Compared with group C, the activity of MSCs cells in group D was increased at 24h, 48h and 72h. There was significant difference between groups (P<0. 05). The apoptosis rates of group A, B, C and D were (4. 23±0. 33), (20. 73±1. 20), (19. 43±1. 16) and (14. 76±0. 80), respectively, and there were differences among groups (F=381. 00, P<0. 001). Compared with group A, the expressions of VEGF and TGF-β₂ in group B were decreased (P<0. 05), and the expressions of VEGF and TGF-β₂ in groups B and C were insignificant (P>0. 05). Compared with group C, the expressions of VEGF and TGF-β₂ in group D were increased, and there were differences among groups (P<0. 05). Luciferase assay confirmed that VEGF and TGF-β₂ were the target genes of Mir-141, and the expressions of VEGF and TGF-β₂ increased when Mir-141 was inhibited. Conclusion: The silencing of Mir-141 can play a protective role of mesenchymal stem cells by improving the activity of bone marrow mesenchymal stem cells in femur head necrosis and inhibiting apoptosis. The mechanism may be related to the activation of VEGF and TGF-β₂ activities.

Key words： Necrosis of the femoral head Bone marrow mesenchymal stem cells miR-141 Vascular endothelial growth factor Transforming growth factor-β₂

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	TIAN Nannan
	FENG Miya
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TIAN Nannan,FENG Miya,et al. Effects of miR-141 on Activity of Bone Marrow Mesenchymal Stem Cells and VEGF/TGF-β₂ Protein in Femoral Head Necrosis[J]. HeBei Med, 2022, 28(12): 1949-1955.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.12.003 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I12/1949

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