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| Protective Effect and Mechanism of Cannabinoid 1 Receptor on and Microglia Activation in Mice with Spinal Cord Injury |
| AN Kang, MA Zhengliang |
| Drum Tower Hospital Affiliated to Medical College Nanjing University, Jiangsu Nanjing 210008, China |
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Abstract Objective: To investigate the protective effect and mechanism of cannabinoid 1 receptor (CB1R) on and microglia activation in mice with spinal cord injury. Methods: Thirty-six female C57BL/6 mice were randomly divided into control group, model group and drug group. The mouse spinal cord injury model was constructed by modified Allen's method. The behavioral evaluation was performed on the 1th, 7th, and 14th day after the operation, respectively. Western blotting was used to detect the protein expression of myelin basic protein (MBP), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in spinal cord tissue. Enzyme-linked immunosorbent assay was used to determine the serum levels of IL-6, IL-17 and TNF-α in mice. Immunofluorescence double staining was used to detect the expression of calcium ion binding regulator (IBA)-1 and ED-1 positive cells in spinal cord tissue. Results: Compared with the control group, the BMS score and the inclination of the oblique plate in the model group were significantly reduced, the serum levels of IL-6, IL-17 and TNF-α, the protein expression of MBP, BNDF and NGF and the number of IBA-1/ED-1 positive activated microglia in the model group was significantly increased (P<0.05). Compared with the model group, the BMS score, the inclination of the oblique plate and the protein expression of MBP, BNDF and NGF in the drug group were significantly increased, the serum levels of IL-6, IL-17 and TNF-αand the number of IBA-1/ED-1 positive activated microglia (P<0.05). Conclusion: CB1R reduces spinal cord injury by inhibiting microglia activation and inflammation.
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2022, Vol. 28 
