干扰Hela细胞TPX2基因对细胞活性及其放疗敏感性的影响研究

doi:10.3969/j.issn.1006-6233.2021.08.02

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Abstract Objective: To analyze the effects of interference with TPX2 gene on the activity and radiotherapy sensitivity of HeLa cells. Methods: Cervical cancer Hela cell is cultured in vitro and divided into interference group, negative control group (NC group), blank control group (CON group), interference and CON group. Respectively the interference group had TPX2-siRNA interference plasmid; CON group had plasmid transfection, replacing the plasmid with PBS; NC group had blank load transfection. CCK 8 experimental detection of efficiency of cell proliferation, Transwell Chambers detection of invasion ability, clone forming experiments to detect radiation sensitivity were all conducted. Results: Compared with the CON group, the relative expression level of TPX2 mRNA, the relative expression level of TPX2 protein, and the cell invasion ability of the NC group did not change significantly (P>0.05); compared with the CON group and the NC group, the TPX2 of the interference group, the relative expression of mRNA, the relative expression of TPX2 protein, and the ability of cell invasion were all significantly reduced (P<0.05). Compared with the CON group, the cell viability of the NC group was not significantly different at each time point (P>0.05); compared with the CON group and the NC group, the culture time of the interference group was 48h, 72h, 96h. The cell viability at time was significantly reduced (P<0.05). Compared with the CON group, the proportion of cell cycles in the G0/G1, G2/M and S phases of the NC group did not change significantly (P>0.05); compared with the NC group, the S and G2/ The proportion of cells in M phase increased significantly, while the proportion of cells in G0/G1 phase decreased significantly (P<0.05). Compared with the CON group, the survival rate of the interference group cells at 4Gy, 6Gy, 8Gy, and 10Gy doses of radiotherapy decreased (P<0.05). Conclusion: Interference with TPX2 gene in cervical cancer Hela cells can effectively interfere with the expression of TPX2 gene and inhibit the cell activity of proliferation and migration of cervical cancer HeLa cells.Interference with TPX2 gene can effectively increase the radiotherapy sensitivity of cervical cancer HeLa cells and improve the curative effect of radiotherapy for cervical cancer.

Key words： Cervical cancer TPX2 gene Cell proliferation

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	QI Xiaoli
	WANG Yuting
	YANG Jie

Cite this article:

QI Xiaoli,WANG Yuting,YANG Jie. Effect of Interference of TPX2 Gene on Cell Activity and Radiotherapy Sensitivity of Hela Cells[J]. HeBei Med, 2021, 27(8): 1239-1243.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.08.02 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I8/1239

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