不同麻醉方式对自发性高血压大鼠脑出血凋亡基因表达水平的影响

doi:10.3969/j.issn.1006-6233.2021.06.01

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Abstract Objective: To explore the effects of different anesthesia methods on neuronal apoptosis and expression of related proteins in spontaneous hypertensive rats with intracerebral hemorrhage. Methods: 30 spontaneously hypertensive rats were used to establish cerebral hemorrhage models by autologous blood injection. They were randomly divided into anesthesia group A and anesthesia group B with 15 rats in each group. Rats in group A were given intravenous inhalation combined with anesthesia, while rats in group B were given target controlled intravenous anesthesia with propofol combined with sufentanil. After the anesthesia took effect, the rats in both groups were injected with autologous blood to build cerebral hemorrhage models. The neurobehavioral scores and brain water content of both groups of rats were observed and compared at 6h, 12h, 24h, 48h and 72h after anesthesia modeling. TUNEL method was used to detect the apoptosis of brain tissue at different time points. The expression of Bax, Bcl-2, caspase-3 and other apoptosis related proteins in brain tissue around intracerebral hemorrhage were detected by immunohistochemistry. The expression of Bax, Bcl-2, caspase-3 and other apoptosis related proteins in brain tissue around intracerebral hemorrhage were detected by RT-PCR and Western blot. Results: Neurobehavioral changes began to appear at 6 hours after intracerebral hemorrhage operation. With the extension of time point, the water content of brain tissue around the hemorrhage area increased significantly, the number of apoptotic cells increased significantly, and the expression of apoptosis related proteins Bax, Bcl-2 and caspase-3 also increased significantly. Compared with anesthesia group A, the nerve of anesthesia group B at 6 h, 12 h, 24 h, 48 h, 72 h after operation was significantly increased. The expression levels of Bax and caspase-3 mRNA and protein were significantly lower than those of group A at the same time point (P<0.05), while the expression of bcl-2 mRNA and protein was significantly higher than that of group A at the same time point (P<0.05). Conclusion: Propofol combined with sufentanil target controlled intravenous anesthesia can reduce the expression of apoptosis related proteins, reduce the apoptosis of nerve cells, reduce the degree of brain level, improve the neurobehavioral score, and play a protective role in the secondary cerebral hemorrhage injury.

Key words： Spontaneous hypertensive rats Intracerebral hemorrhage Apoptosis Target controlled intravenous anesthesia

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.06.01 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I6/881

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