UHRF1在三阴乳腺癌细胞中的表达及增殖侵袭的影响

doi:10.3969/j.issn.1006-6233.2021.05.01

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Abstract Objective: To study the expression of UHRF1 in triple negative breast cancer (TNBC) breast tissue and its cell line and its effect on cancer cell proliferation and invasion. Methods: Immunohistochemistry was used to detect the expression of UHRF1 in breast tissues of non-TNBC and TNBC patients; immunofluorescence was used to detect the localized expression of Ki67 and UHRF1 in breast tissues of non-TNBC and TNBC patients; qRT-PCR and Western blot were used to detect untransformed human breast epithelial cells (MCF-10A), estrogen and progesterone receptor (ER+ / PR+) breast cancer cell lines (MCF-7) and TNBC breast cancer cell lines (MDA-MB-231 and MDA-MB-436) UHRF1 mRNA and protein expression ;UHRF1-shRNA lentivirus was used to transduce TNBC cell lines (MDA-MB-231 and MDA-MB-436) and the UHRF1 knock down (Knock down, UHRF1 KD) cell line was constructed; Ki67 staining and CCK-8 were used to detect WT and UHRF1 KD cell proliferation and viability changes; Transwell was used to detect WT and UHRF1 KD cell invasion changes; qPCR was used to detect the expression of FOXO4, PPARG, P53 and P21 mRNA. Results: The expression of UHRF1 in breast tissue of TNBC patients was significantly higher than that in non-TNBC patients (P<0.05); the ratio of UHRF1+Ki67+/Ki67+ cells in breast tissue of TNBC patients was significantly higher than that in non-TNBC patients (P<0.05); TNBC cell line ( MDA-MB-231 and MDA-MB-436) UHRF1 mRNA and protein expression were significantly higher than human breast epithelial cells (MCF-10A) and estrogen and progesterone receptor (ER+ / PR+) breast cancer cell lines (MCF- 7) (P<0.05); compared with WT's TNBC cell line (MDA-MB-231 and MDA-MB-436), UHRF1 KD's TNBC cell line (MDA-MB-231 and MDA-MB-436) cells Proliferation, vitality and invasion ability were significantly reduced (P<0.05); UHRF1 KD TNBC cell lines (MDA-MB-231 and MDA-MB-436) FOXO4, PPARG, P53 and P21 mRNA expression were significantly reduced (P<0.05) . Conclusion: UHRF1 is highly expressed in TNBC breast tissues and cell lines, and down-regulation of UHRF1 can inhibit the proliferation and invasion of TNBC cells, which is related to the inhibition of FOXO4/P53 and PPARG/P21 signaling pathways.

Key words： Triple-negative breast cancer UHRF1 Cell proliferation Invasion

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	YUAN Huiling
	WU Lihua
	CHEN Guilin
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Cite this article:

YUAN Huiling,WU Lihua,CHEN Guilin, et al. Expression of UHRF1 in Triple-negative Breast Cancer Cells and Its Effect on Proliferation and Invasion[J]. HeBei Med, 2021, 27(5): 705-710.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.05.01 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I5/705

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