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| Preparation of Puerarin Combined with Tricalcium Phosphate Scaffold and Its Repairing Effect on Steroid-Induced Avascular Necrosis of the Femoral Head in Rats |
| TANGg Hua, et al |
| Sichuan Modern Hospital, Sichuan Chengdu 610000, China |
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Abstract Objective: To prepare puerarin/tricalcium phosphate scaffold material,observe its cell compatibility,and test its effect on bone repair in rats with steroid-induced avascular necrosis of the femoral head. Methods: Using puerarin and tricalcium phosphate as materials,the puerarin/tricalcium phosphate scaffold material was prepared according to the method of negative pressure adsorption. The surface morphology of the scaffold material was observed by scanning electron microscope,and the compressive strength and porosity were detected,as well as the effects of different concentrations of puerarin/tricalcium phosphate scaffold material on the activity and growth of bone cells MLO-Y4. 60 SD rats were randomly divided into blank control group,model group and composite scaffold group,20 rats in each group. In the model group and the composite stent group,2 mg/kg LPS was injected into the tail vein once a day for 2 days. After the last injection of LPS,20 mg/kg methylprednisolone was injected intraperitoneally,once a day for 3 days,to build a femoral head necrosis model. After modeling,rats in the composite scaffold group were implanted with puerarin/tricalcium phosphate scaffold material; after 12 weeks,the blood of rats was collected,and the content of calcium and phosphorus in serum samples was detected by an automatic biochemical analyzer. ELISA kits were used to detect the content of bone alkaline phosphatase (BALP) and osteocalcin (BGP). Micro-CT scanners were used to detect the bone mineral density (BMD) and trabecular bone volume fraction (Tb.BV/TV),trabecular bone thickness (Tb.Th) and trabecular bone number (Tb.N). Hematoxylin-eosin staining (HE) was used to observe the histopathological changes of femur in each group. Immunohistochemical staining was used to detect the expression of VEGF and Runχ2 in femoral tissue. Results: The prepared puerarin/tricalcium phosphate scaffold material has a porous network structure,the compressive strength increases,and the porosity decreases; the puerarin/tricalcium phosphate scaffold material extract can increase the activity of MLO-Y4 cells. Compared with the control group,the femoral tissue of the model group showed obvious necrosis,BMD decreased,Tb.BV/TV,Tb.Th and Tb.N decreased,serum calcium and phosphorus levels decreased,and BALP levels increased,BGP level decreased,expression of VEGF and Runχ2 in femoral tissue decreased. Compared with the model group,the bone sclerosis of rats in the composite scaffold group was significantly improved,BMD increased,and Tb.BV/TV,Tb.Th and Tb.N increased,serum calcium and phosphorus levels increased,BALP The level decreased and BGP level increased,and the expression of VEGF and Runχ2 in femoral tissue increased. Conclusion: The puerarin/tricalcium phosphate scaffold material has a good repairing effect on the necrotic femur of hormonal femoral head necrosis rats.
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2021, Vol. 27 
