ATF2在宫颈癌组织中的表达及沉默ATF2抑制自噬对宫颈癌细胞化疗敏感性的影响

doi:10.3969/j.issn.1006-6233.2021.02.007

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Abstract Objective: To investigate the expression of ATF2 in cervical cancer tissues and the effect of silencing ATF2 on the sensitivity of cervical cancer cells to the inhibition of autophagy. Methods: Immunohistochemical method was used to detect the expression of ATF2 in cervical cancer tissues; qRT-PCR and Western blot were used to detect the expression of ATF2 in cervical cancer tissues and cervical cancer cell lines; Cell clone formation experiment was used to detect the proliferation ability of HeLa cells; Western blot was used to analyze of the ratio of LC3 II/LC3 I protein expression levels in HeLa cells; Cellular immunofluorescence staining was used to detect the number of autophagosomes in HeLa cells; Cell flow cytometry was used to detect the apoptosis rate of HeLa cells; Western blot was used to detecte the expression of Bax and Bcl-2 protein in HeLa cells. Results: ATF2 was highly expressed in cervical cancer tissues and cervical cancer cell lines (P<0.01); Silent ATF2 combined with DDP reduced the proliferation capacity of HeLa cells (P<0.001), the ratio of LC3 II/LC3 I protein expression level (P<0.01) and the number of autophagosomes (P<0.01); Silent ATF2 combined with DDP increased the apoptosis rate of HeLa cells (P<0.01), Bax protein expression level was up-regulated (P<0.001), Bcl-2 protein expression level was down-regulated (P<0.001). Conclusion: Atf2 is highly expressed in cervical cancer tissues and cervical cancer cells. Silencing Atf2 inhibits autophagy, enhances the sensitivity of HeLa to DDP, and increases the apoptosis rate, thus enhancing the chemosensitivity of HeLa cells.

Key words： Cervical cancer ATF2 Autophagy Chemotherapy sensitivity

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	ZHOU Zhan
	LI Yi

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ZHOU Zhan,LI Yi. Expression of ATF2 in Cervical Cancer Tissues and the Effect of Silencing ATF2 to Inhibit Autophagy on Chemotherapy Sensitivity of Cervical Cancer Cells[J]. HeBei Med, 2021, 27(2): 207-211.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.02.007 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I2/207

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