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| The Expression and Correlation of MiR-29a MiR-126a-5p and MiR-21-3p in Severe Acute Pancreatitis |
| ZHANG Qianqian, WANG Chengli, Fu Tian, et al |
| 3201 Hospital, Shaanxi Hanzhong 723000, China |
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Abstract Objective: To explore the expression and correlation of microRNA-29a (miR-29a), microRNA-126a-5p (miR-126a-5p) and microRNA-21-3p (miR-21-3p) in severe acute pancreatitis. Methods: A total of 82 patients with acute pancreatitis in our hospital from March 2020 to March 2021 were selected as the observation group, including 43 patients with mild acute pancreatitis, 39 patients with severe acute pancreatitis, and 40 normal physical examinees in the same period were selected as the control group. The expressions of miR-29a, mir-126a-5p and mir-21-3p were detected by real-time fluorescence quantitative method. The severity of patients was evaluated by acute physiology and chronic health assessment (APACHE II) and Ranson scoring system. Pearson correlation was used to analyze the correlation of miR-29a, mir-126a-5p and mir-21-3p. Results: Compared with the control group, the expression levels of miR-29a and miR-21-3p in the observation group increased, and the expression levels of miR-126a-5p decreased, with statistical significance (P<0.05). Compared with patients with mild acute pancreatitis, the expression levels of miR-29a and miR-21-3p reduce in patients with severe acute pancreatitis, and the expression levels of miR-126a-5p rise, with statistical significance (P<0.05). With the exacerbation of severe acute pancreatitis, the expression levels of miR-29a and miR-21-3p increased, and the expression levels of miR-126a-5p decreased, with statistical significance (P<0.05). The results show that the negative phase of miR-29a is related to mir-126a-5p (r=-0.447; P = 0.003); the positive correlation of miR-29a with mir-21-3p (r=0.534; P = 0.001);the negative phase of mir-126a-5p was related to mir-21-3p (r=-0.517; P = 0.001). Conclusion: MiR-29a and miR-21-3p are highly expressed in the serum of patients with severe acute pancreatitis, and miR-126a-5p is low expressed in the serum of patients with severe acute pancreatitis, and abnormal levels of miR-29a, miR-126a-5p and miR-21-3p were risk factors for severe acute pancreatitis. It can be used as an indicator for the detection of severe acute pancreatitis.
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2021, Vol. 27 
