转录因子EZH2和RUNX3在骨巨细胞瘤中的表达及与复发的关系研究

doi:10.3969/j.issn.1006-6233.2021.10.003

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Abstract Objective: To investigate the expression of zeste homolog 2 (EZH2) and human RUNT-related transcription factor 3 (RUNX3) in giant cell tumor of bone and its relationship with recurrence of giant cell tumor of bone. Methods: Fifty-three patients who were diagnosed with giant cell tumor of bone and were treated by surgical examination in our hospital from October 2013 to January 2018 were selected as the research subjects, and the tumor tissues and adjacent tissues (> 3 cm from the tumor tissue) of patients with giant cell tumor of bone were collected during surgery, the expressions of EZH2 and RUNX3 were detected by immunohistochemistry, and their relationships with the clinicopathological characteristics of patients were analyzed; Spearman was used for correlation analysis; and after 36 months follow-up, the expressions of EZH2 and RUNX3 were compared between the relapse group and the non-relapse group. Results: The positive expression rate of EZH2 (69.81%) in giant cell tumor of bone was higher than that in adjacent tissues (15.09%) (P<0.05), and the positive expression rate of RUNX3 (32.08%) was lower than that in adjacent tissues (86.79%) (P<0.05); the expressions of EZH2 and RUNX3 in the tumor tissues were related to tumor diameter, imaging grade, pathological grade and degree of infiltration (P<0.05); Spearman correlation analysis showed that the expressions of EZH2 and RUNX3 were negatively correlated in giant bone tumor tissue (r = -0.544, P<0.05); the positive expression rate of EZH2 in recurrent group (93.33%) was higher than that in non-recurrent group (60.53%) (P<0.05), and the positive expression rate of RUNX3 in recurrent group (0.00%) was lower than that in non-recurrent group (44.74%) (P<0.05). Conclusion: EZH2 is highly expressed in giant cell tumor of bone, and RUNX3 is lowly expressed, which is closely related to the clinicopathological characteristics of patients. The expressions of EZH2 and RUNX3 are negatively correlated, EZH2 may inhibit the expression of RUNX3, and EZH2 and RUNX3 may be used for the evaluations of the disease and prognosis of giant cell tumor of bone.

Key words： Enhancer of zeste homolog 2 Human RUNT-related transcription factor 3 Giant cell tumor of bone

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.10.003 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I10/1598

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