穿龙薯蓣皂苷元对CIA小鼠脾脏Treg细胞的影响

doi:10.3969/j.issn.1006-6233.2021.01.004

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Abstract Objective: To evaluate the effect of diosgenin on regulatory Treg cells in spleen of collagen induced arthritis (CIA) mice. Methods: The CIA mice model was established by immunizing DBA1/J mice with chicken type II collagen and Freund’s completed adjuvant. And CD4⁺CD25⁺Treg cells in spleen of CIA model mice were isolated by MACS. Then, to detect the purity of Treg cells by flow cytometry and culture them in vitro. 48 hours after treatment, the proportion of Treg cells was detected by flow cytometry, the expression of transcription factor Foxp3 in Treg cells was detected by real-time fluorescence quantitative PCR, and the levels of IL-10 were detected by ELISA. Results: CIA model mice were established successfully. The purity of Treg cells isolated by MACS was more than 90%. Compared with the control group, the percentage of Treg cells, the Foxp3 mRNA level, and the expression of IL-10 in cell supernatant were increased significantly (P<0.05) 48 hours after treatment.Conclusion: Diosgenin can significantly increase the proportion of Treg cells, the levels of transcription factor Foxp3 and IL-10 in CIA mice.

Key words： CIA model Regulatory T cells Foxp3 Interleukin-10 Diosgenin

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.01.004 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I1/15

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