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| Levels and Clinical Significance of Peripheral Blood Mononuclear Cells Treg and Th17 Cells before and after treatment of Severe Bronchiolitis in Children |
| WANG Wenhui, LI Minfu, WANG Ru, er al |
| Qionghai People's Hospital, Hainan Qionghai 571400, China |
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Abstract Objective: To explore the changes of levels of peripheral blood mononuclear cells Treg and Th17 cells before and after treatment of severe bronchiolitis in children. Methods: A total of 106 children patients with severe bronchiolitis who were admitted between September 2017 and September 2018 were selected for the study. All patients were closely monitored for disease changes, and given symptomatic supportive therapy, antiviral therapy and aerosol inhalation of budesonide or ipratropium bromide. The efficacy was evaluated in the two groups, and the levels of Treg and Th17 and related cytokines [interleukin-17 (IL-17), interleukin-23 (IL-23), interleukin-10 (IL-10), transforming growth factor-β (TGF-β)] were compared before treatment and after 1w of treatment, and the levels of Treg and Th17 were evaluated in children patients with different efficacy. Spearman correlation analysis was used to analyze the correlation between efficacy and Treg and Th17 levels after treatment. Results: After 7d of treatment, the level of Treg was higher than that before treatment (P<0.05) while the level of Th17 was lower than that before treatment (P<0.05). After 7d of treatment, the levels of IL-10 and TGF-β were higher than those before treatment (P<0.05) while the levels of IL-17 and IL-23 were lower than those before treatment (P<0.05). After 7d of treatment, the Treg levels in markedly effective children patients and effective children patients were higher than those before treatment (P<0.05) while the Th17 levels were lower than those before treatment (P<0.05).There were no significant differences in the levels of Treg and Th17 of ineffective children patients compared with those before treatment (P>0.05) There were statistically significant differences in the levels of Treg and Th17 after treatment among markedly effective, effective and ineffective children patients (P<0.05). Conclusion: The levels of Treg and its cytokines in children patients with severe bronchitis after treatment are higher than those before treatment, and Th17 cytokines are lower than those before treatment, and the efficacy of children patients is related to the balance of Th17/Treg.
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2020, Vol. 26 
