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| Effects of Notch Signaling Pathway on Proliferation Invasion and Migration of Prostate Cancer PC-3 cells |
| LI Chuangui, WANG Qiang |
| Baoding First Central Hospital of Hebei Province, Hebei Baoding 071000, China |
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Abstract Objective: To investigate the effects and the mechanism of DAPT blocking and NICD adenovirus activating Notch signaling pathway on proliferation, invasion and migration of prostate cancer PC-3 cells and its possible mechanism. Methods: Efficiency of DAPT blocking and AdNICD activating the Notch signaling pathway was detected by real-time PCR. EdU assay was used to evaluate the cell proliferation. Transwell assay and wound healing assay were used to detect the invasion and migration ability of PC-3 cells, respectively. p-AKT protein expression was detected by using western blot assay. Results: DAPT effectively blocked Notch signaling pathway. After adding DAPT to prostate cancer PC-3 cells, the proliferation, invasion and migration ability of cells decreased significantly, and the phosphorylation level of AKT reduced obviously. Infection of NICD adenovirus effectively activated Notch signaling pathway. After adding AdNICD to prostate cancer PC-3 cells, the proliferation, invasion and migration ability to cells increased significantly, and the phosphorylation level of AKT protein raised evidently. The difference was statistically significant. Conclusion: Notch signaling pathway can affect the proliferation, invasion and migration of PC-3 cells by regulating Akt signaling pathway.
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2020, Vol. 26 
