IL-8基因型多态性及其表达水平对鼻咽癌易感性的诊断价值

doi:10.3969/j.issn.1006-6233.2020.04.001

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Abstract Objective: To analyze the interleukin-8 (IL-8) genotype polymorphism and its expression level in NPC patients, and to explore its diagnostic value in NPC susceptibility. Methods: A total of 168 NPC patients in our hospital from January 2017 to September 2019 were collected as the experimental group. 232 healthy patients in the same period were selected as the control group (NC group). The polymorphism of IL-8 gene - 251, + 781, + 1633 and + 2767 were analyzed by PCR-RFLP, and the serum IL-8 in NPC patients was detected by ELISA The relationship between IL-8 gene polymorphism and NPC susceptibility was evaluated by logistic regression analysis. The interaction of different exposure factors in NPC susceptibility was analyzed. The diagnostic value of IL-8 level in NPC was evaluated by ROC. Results: Compared with the NC group, the risk of NPC in the IL-8-251 gene locus TT genotype (OR=1.98, 95%CI: 1.00 ~ 3.82) and AA genotype (OR=1.76, 95%CI: 1.02 ~ 3.08) was significantly increased, the risk of NPC in the TA genotype (OR=1.35, 95%CI: 1.01~2.61) was significantly reduced, and the expression level of IL-8 in NPC patients was significantly increased (P<0.05); There was a negative interaction between IL-8-251 SNPs and smoking and EBV infection factors in the occurrence of NPC. The area under the curve (AUC) of IL-8 susceptibility to NPC was 0.852, with 86% sensitivity and 78% specificity. Conclusion: The genotype polymorphism at the -251 locus of IL-8 gene and increased expression of serum IL-8 are related to NPC susceptibility, and interact with smoking and EBV infection exposure. IL-8 level is of diagnostic value for NPC susceptibility.

Key words： IL-8 Gene polymorphism Nasopharyngeal carcinoma

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.04.001 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I4/529

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