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| Study on the Effect of ANP on the Proliferation and Migration of Gastric Cancer MGC-803 Cells and Its Mechanism |
| ZHU Yaqing, LI Chunhui, et al |
| The Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the in vitro effect of atrial natriuretic peptide (ANP) on human gastric cancer cell line MGC-803 and the combined effect of PI3K/Akt signaling pathway inhibitor (LY294002) on human gastric cancer cell line MGC-803 and the possible mechanism. Methods: The gastric cancer MGC-803 cells were treated with different concentrations of ANP and different concentrations of LY294002 .The cell proliferation inhibition rate was detected by MTT assay. MGC-803 cells were randomly divided into four groups: control group, ANP group , LY294002 group and ANP combined LY294002 group. CCK8 was used to detect cell proliferation, scratch test was used to detect cell migration, cell morphology was observed under an inverted microscope and Western blot was used to detect the expression of key signal molecules total Akt (t-Akt) and p-Akt protein of PI3K/Akt signaling pathway. Results: CCK8 results showed that compared with control group, ANP group and LY294002 group, the combination of ANP and LY294002 can inhibit the proliferation of MGC-803 cells, with statistically significant differences(P<0.05); the inhibition of ANP alone and LY294002 alone on the proliferation of MGC-803 cells was time-dependent and dose-dependent. The 24-hour scratch area repair rate of the control group, ANP group, LY294002 group and ANP combined LY294002 group were (50.99±1.80)%, (43.33±2.22)%,(35.81±4.09)%,(26.67±1.69)% and the difference was statistically significant (P<0.05). Four groups of MGC-803 cells showed different cell morphology changes. Both ANP and LY294002 decreased the expression level of p-Akt protein in MGC-803 cells, and ANP combined with LY294002 had a more significant inhibitory effect on the expression of p-Akt protein than they were used alone (P<0.05). The expression level of t-Akt had no significant change in the four groups of cells (P>0.05). Conclusion: ANP can inhibit the proliferation and migration of human gastric cancer cell MGC-803. The combination of ANP and LY294002 can increase the inhibitory effect on gastric cancer cell MGC-803. The mechanism may be related to the inhibition of PI3K/Akt signaling pathway.
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2020, Vol. 26 
