|
|
|
| Concentrated Growth Factor Promotes Angiogenic Potential of Endothelial Cells in Vitro |
| LI Shiyan, MA Zhihong, LI Yanbo, et al |
| Chengde Central Hospital,Hebei Chengde 067000,China |
|
|
|
|
Abstract Objective: To study the effect of concentrated growth factor (CGF) on the apoptosis of MLO-Y4 osteocyte-like cells.Methods: CGF was prepared from the venous blood of healthy volunteers.The model of ischemia-reperfusion in vitro was duplicated and divided into control and experimental groups with different concentrations (including 1%CGFe,5% CGFe,10% CGFe).The expression levels of Bax,Bcl-2 and p53 mRNA were detected by real-time fluorescence quantitative polymerase chain reaction (qRT PCR) to evaluate the degree of MLO-Y4 osteocyte-like cells apoptosis.Results: CGF could significantly inhibit the increase of caspase-3 activity of MLO-Y4 osteocyte-like cells induced by ischemia-reperfusion,with the decrease of Bax,Bcl-2 and p53 mRNA levels.Conclusion: CGF can improve the apoptosis of human umbilical vein endothelial cells induced by ischemia-reperfusion.
|
|
|
|
|
|
[1] Gomez-Barrena E,Rosset P,Lozano D,et al.Bone fracture healing:cell therapy in delayed unions and nonunions[J].Bone,2015,70:93~101.
[2] Lukac N,Katavic V,Novak S,et al.What do we know about bone morphogenetic proteins and osteochondroprogenitors in inflammatory conditions[J].Bone,2020,137:115403.
[3] Bonazza V,Borsani E,Buffoli B,et al.In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines[J].Cell Biol Int,2018,42:353~364.
[4] Qin J,Wang L,Sun Y,et al.Concentrated growth factor increases Schwann cell proliferation and neurotrophic factor secretion and promotes functional nerve recovery in vivo[J].Int Mol Med,2016,37:493~500.
[5] Dang M,Zeng X,Chen B,et al.Interferon-gamma mediates the protective effects of soluble receptor for advanced glycation end-product in myocardial ischemia/reperfusion[J].Lab Invest,2019,99:358~370.
[6] Qiao J,An N and Ouyang X.Quantification of growth factors in different platelet concentrates[J].Platelets,2017,28:774~778.
[7] Chandra A,Lin T,Young T,et al.Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone-Forming Cells and Their Progenitors Through Distinct Mechanisms[J].Bone Miner Res,2017,32:360~372. |
|
|
|
2020, Vol. 26 
