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Study on the Role and Mechanism of Beclin1-Regulated Autophagy in Embryo Fragment Formation |
NIU Huanfu, NIU Shufang, WEI Zefeng, et al |
Reproductive Medicine Centre, Affiliated Hospital of Jining Medical University, Shandong Jining 272029, China |
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Abstract Objective: To study the role and possible mechanism of beclin1-regulated autophagy in embryo fragment formation. Methods: A total of 240 patients who underwent in vitro fertilization of eggs in our hospital from July 2016 to October 2019 were selected. Embryo (3PN) was selected and subdivided into the following four groups according to different fragment contents: low fragment group (fragment proportion <10%), medium and low fragment group (25%> fragment proportion ≥10%), medium and high fragment group (50%> fragment proportion ≥25%), and high fragment group (fragment proportion ≥50%), with 60 cases in each group. The selected embryos were cultured in G2 blastocyst medium and interferon (40ng/mL) interferon was used for 36h. Beclin1, PI3K, LC3B, mTOR and AKT were quantitatively determined by western blot. Results: Before and after interference, Beclin1 protein, PI3K protein, LC3B protein, mTOR protein and AKT protein content in the four groups were significantly increased, with statistically significant differences (P<0.05). Before interference, the contents of the six proteins were compared, from high to low: low fragment group, medium and low fragment group, medium and high fragment group, and high fragment group, with statistically significant differences (P<0.05). After infection, the contents of Beclin1 protein, PI3K protein and LC3B protein in the four groups were significantly different (P<0.05). Beclin1 was significantly correlated with PI3K, LC3B, mTOR and AKT (P<0.05). Conclusion: A high proportion of embryo fragments will inhibit Beclin1, PI3K, LC3B, mTOR and AKT protein content. Beclin1's regulation of autophagy may produce interferon-like effects, thus affecting the expression of related channel proteins.
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