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| Effects of β-carboline Alkaloids on SGC-7901 Tumor Bearing Mice through FAK/PI3K/AKT/mTOR Pathway |
| FAN Yuxiang, ZENG Fanye, ZHANG Hongliang |
| The Fourth Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi 830000, China |
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Abstract Objective: To investigate the effect of β-carboline alkaloids extracted from Peganum harmala L. on the expression of FAK, PI3K, AKT, mTOR, Bcl-2 and Bax in transplanted tumor tissue of human gastric cancer SGC-7901 mice. Methods: The transplanted tumor model was established in mice. 50 Kunming mice were randomly divided into 5 groups: blank control group, 5-fluorouracil group, low-, medium-and high-dose β-carboline alkaloids groups, with 10 mice in each group. The tumor inhibition rate was calculated, the pathological changes of tumor tissues were detected by HE staining, the apoptosis of tumor cells was detected by TUNEL method, the mRNA expression of FAK, PI3K, Akt, mTOR, Bcl-2 and Bax were detected by RT-PCR The expression of FAK, PI3K, Akt, mTOR, Bcl-2 and Bax were detected by blot and immunohistochemistry. Results: The middle and high dose of β-carboline alkaloids can inhibit the growth of SGC-7901 mice tumor and reduce the tumor weight (P<0.01), which is better than the low dose of β-carboline alkaloids (P<0.05). He staining and TUNEL results showed that β-carboline alkaloids could damage the gastric tumor cells and induce apoptosis. RT-PCR, Western blot and immunohistochemistry showed that the middle and high doses of β-carboline alkaloids could significantly reduce the expression of FAK, PI3K, AKT, mTOR and Bcl-2 protein (P<0.01), and increase the expression of Bax protein (P<0.01), which was better than the low doses of β-carboline alkaloids (P<0.05) and the difference was statistically significant. Conclusion: The inhibitory effect of β-carboline alkaloids on gastric cancer is related to the decrease of Bcl-2, FAK, PI3K, AKT, mTOR protein expression and the increase of Bax protein expression, which indicates that β-carboline alkaloids may be a potential therapeutic drug for gastric cancer.
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2020, Vol. 26 
