|
|
|
| The Diagnosis Valne of Combined Detection of Pepsinogen I Gastrin-17 and TOLL-like Receptors in Peripheral Blood in Early Laryngeal Cancer |
| ZHANG Yi, WANG Zhiping, BAI Jie, et al |
| Harrison International Peace Hospital, Hebei Hengshui 053000, China |
|
|
|
|
Abstract Objective: To investigate the value of combined detection of pepsinogen-I (PG-I), gastrin-17 (G-17) L and Toll-like receptors (TLR4) in peripheral blood in the diagnosis of early laryngeal cancer. Methods: 270 cases of suspected laryngeal cancer and 90 cases of health check-up were selected from January 2013 to May 2017 in our hospital. The levels of PG-Ⅰ and G-17 in serum of the three groups were detected by enzyme linked immunosorbent assay (ELISA), and (peripheral blood mononuclear cell, in peripheral blood mononuclear cells (PBMC) was measured by real-time fluorescence quantitative polymerase chain reaction (real-time polymerase chain reaction,RT-PCR). TLR4 mRNA expression in PBMC. Results: The level of PG-I in vocal cord polyp group was significantly lower than that in healthy control group (P < 0.05), while that in laryngeal cancer group was much lower than that in other two groups (P < 0.05); there was no significant difference between healthy control group and vocal cord polyp group (P > 0.05), and the level of G-17 in laryngeal cancer patients was significantly higher than that in healthy control group and vocal cord polyp group (P < 0.05). The expression of TLR4 in cancer group was significantly higher than that in vocal cord polyp group and polyp group, and the difference was statistically significant (P < 0.05). The sensitivity and negative predictive value of combined detection of three indicators were significantly higher than that of single indicator detection (P < 0.05). Conclusion: The concentration of PGI and G-17 in peripheral serum and the expression of TLR4 in PBMC are of great significance for the diagnosis and differentiation of early laryngeal cancer. The combined detection method can significantly improve the diagnostic efficiency.
|
|
|
|
|
|
[1] 席盼盼,张淑香.凋亡相关基因及其与喉癌关系的研究进展[J].蚌埠医学院学报,2016,41(1):137~140.
[2] 曾珍,苏跃,王中亮.全喉切除术后喉发音功能重建的研究进展[J].中国眼耳鼻喉科杂志,2016,16(1):53~56.
[3] 刘泽阳,许洪波,郭伟,等.胃蛋白酶在咽喉反流与喉癌中的临床意义及研究进展[J].临床耳鼻咽喉头颈外科杂志,2018,21(7):559~562.
[4] 龚霞,秦永,王晓云.喉癌组织中胃蛋白酶表达的初步研究[J].听力学及言语疾病杂志,2014,22(4):375~377.
[5] 存在食管外症状的胃食管反流病患者的临床特点及反流监测的研究[D].河北医科大学,2016.
[6] 章武战,章国东,陈俊华.胃蛋白酶原Ⅰ、Ⅱ测定在胃癌早期诊断中的研究[J].中国卫生检验杂志,2014,24(12):1718~1719.
[7] Mansour-Ghanaei F,Joukar F,Rajpout Y,et al.Screening of precancerous gastric lesions by serum pepsinogen, gastrin-17, anti-helicobacter pylori and anti-caga antibodies in dyspeptic patients over 50 years old in guilan province, north of Iran[J].Asian Pac Cancer Prev,2014,15(18):7635~7638.
[8] 严波,杨善峰,李祥,等.血清胃泌素17、胸苷激酶1联合检测辅助诊断胃癌的价值[J].山东医药,2016,56(8):40~41.
[9] Volk-Draper L,Hall K,Griggs C,et al.Paclitaxel therapy promotes breast cancer metastasis in a TLR4-dependent manner[J].Cancer Res,2014,74(19):5421~5434.
[10] 汤夏冰,李蕾,庄强尔,等.TLR4/NF-κB通路在脂多糖诱导喉癌细胞释放HMGB1中的作用[J].江苏医药,2013,39(15):1762~1764. |
|
|
|
2019, Vol. 25 
