原发性痛风性关节炎患者miR-23a miR-24-2和miR-27a的表达水平及调节机制

doi:10.3969/j.issn.1006-6233.2019.08.001

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Abstract Objective: To analyze the expression level and regulation mechanism of microRNA-23a (miR-23a), microRNA-24-2 (miR-24-2) and microRNA-27a (miR-27a) in patients with primary gouty arthritis (PGA). Methods: 50 patients with PGA in our hospital were selected as case group and 30 health physical examination personnel as control group. The expression levels of miR-23a, miR-24-2 and miR-27a were detected by real-time fluorescent quantitative PCR. Pearson correlation analysis was used to analyze the relationship between the expressions of miR-23a, miR-24-2 and miR-27a. The data were included in statistical analysis. Results: The plasma fasting blood glucose, uric acid, white blood cell count, neutrophils, triglycerides and apolipoprotein B100 in the case group were significantly higher than those in the control group (all P<0.05), apolipoprotein A1 was significantly lower than those in the control group (P<0.01), but there was no significant difference in total cholesterol between the two groups (P>0.05). Compared with the control group, the expression levels of miR-23a, miR-24-2 and miR-27a in the case group were significantly decreased (P<0.01). Pearson correlation analysis showed that there was a positive correlation between the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells of patients (P<0.01). Urate (100 g/ml) was used to stimulate peripheral blood mononuclear cells in healthy people. The results showed that the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells in the stimulation group were significantly lower than those in the non-stimulation group (P<0.01). Conclusion: Mi-23a, mir-24-2 and mir-27a may play an important role in the inflammatory immune response of PGA as negative inflammatory regulators, and mir-24-2 may regulate the lipoprotein of PGA.

Key words： Primary gouty arthritis MicroRNAs Mi-23a-27a-24-2 clusters Peripheral blood mononuclear cells Real-time fluorescence quantitative PCR

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2019.08.001 OR http://www.hbyxzzs.cn/EN/Y2019/V25/I8/1233

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