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| Expression and Correlation of Mismatch Repair Proteins and Epithelial-mesenchymal Transition Markers in Colorectal Cancer |
| WU Jianlong, LIU Jianwei, LIU Haili, et al |
| Chengde Central Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the expression and significance of mismatch repair protein (MMR) and epithelial-mesenchymal transition (EMT) markers in colorectal cancer, and to analyze the relationship between them. Methods: Immunohistochemistry was used to label the expression of mismatch repair proteins (MSH1, MSH2, MSH6, and PMS2) and epithelial-mesenchymal transition markers (E-cadherin, Vimentin) in colorectal cancer tissues. One or more of the four proteins of MSH1, MSH2, MSH6, and PMS2 were identified as mismatch repair gene defects (dMMR), and all expressions were judged to be mismatched gene integrity (pMMR). Results: 1 Among the 131 patients with colorectal cancer, 112 were pMMR, and the MMR protein expression rate was 85.50%. In 19 cases of dMMR, the MMR protein deletion rate was 14.50%. According to the analysis, in patients with colorectal cancer, dMMR is partially different from the disease site. In 32 patients with right colon cancer, there are 12 cases of dMMR, accounting for 37.5%, and among the 71 cases of left colon cancer, dMMR is common. In 4 cases, the proportion was 5.6%. In 28 cases of rectal cancer, there were 3 cases of dMMR, accounting for 10.7%. And in the lymph node metastasis group, the dMMR in the distant metastasis group and the invasive muscular layer group was significantly lower than that in the no lymph node metastasis group, the no distant metastasis group, and the invasive muscular layer group (P<0.05). The expression of E-cadherin in colorectal cancer was 2131 cases. 115 cases (86.68%) were significantly lower than adjacent tissues (99.24%), and Vimentin expression was significantly higher in 21 cases (16.03%) than in adjacent tissues (0%) (P<0.05). The expression of E-cadherin in poorly differentiated colorectal cancer, lymph node group and invasive muscle layer was much lower than that in high-medium differentiated colorectal cancer, no lymph node group, no distant metastasis and invasion of muscle layer (P<0.05). In contrast, the expression of Vimentin was reversed (P< 0.05). The expression of E-cadherin in the right colon cancer was higher than that in the left colon and rectal cancer. The expression of Vimentin right colon cancer was lower than that of left colon cancer and rectal cancer (P<0.05). Conclusion: The status of mismatch repair protein and epithelial-mesenchymal transition are related to the occurrence and progression of colorectal cancer, and affect its prognosis. It also suggests that the colorectal cancer with insignificant epithelial-mesenchymal transition has a high MMR protein deletion rate and mismatch. Repairing genetically defective colorectal cancer will have a better prognosis.
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2019, Vol. 25 
