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| Expression of lncRNA-CECR7 in Esophageal Cancer Cells and its Effect on Expression of CDK4, Cyclin D1 and PAK2 Genes |
| DU Fang, et al |
| 988 Hospital of Joint Logistics Support Force, Henan Zhengzhou 450042, China |
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Abstract Objective: To investigate the expression of long non-coding RNA-CECR7 (lncRNA-CECR7) in esophageal cancer cells and its effect on the expression of p2l-activated kinase 2 (PAK2), Cyclin D1 (Cyclin D1) and cyclin-dependent kinase 4 (CDK4) genes. Methods: The expression of lncrna-cecr7 in human esophageal cancer cells Eca109, EC9706, kyse30, TE-13 and het-1a were detected by real-time fluorescence quantitative PCR. The esophageal cancer cells with the highest expression were transfected, and the control group (siRNA of negative control) and the intervention group (siRNA of lncrna-cecr7) were set up respectively. The expression of lncrna-cecr7 was detected by qRT-PCR, the apoptosis rate was detected by flow cytometry, and the expression of PAK2, cyclin D1 and CDK4 were detected by qRT-PCR. Results: Compared with het-1a, the expression of lncrna-cecr7 in esophageal cancer cells was significantly up-regulated (P<0.01), and the expression of lncrna-cecr7 was the highest in TE-13 cells. The expression of lncrna-cecr7 in TE-13 cells in the intervention group was significantly lower than that in the control group (P<0.01). After silencing lncrna-cecr7, the apoptosis rate of TE-13 cells in the intervention group was significantly higher than that in the control group (P<0.01). The expression of PAK2, cyclin D1 and CDK4 mRNA in the intervention group was significantly lower than that in the control group (P<0.01). Conclusion: lncRNA-CECR7 is highly expressed in esophageal cancer cells, which can induce apoptosis by silencing lncRNA-CECR7, and its mechanism may be closely related to the down-regulation of PAK2, Cyclin D1 and CDK4 gene expressions.
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2019, Vol. 25 
