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| Effects of Matrine Preconditioning on Th1/Th2 Cytokines in Peripheral Blood of Rats with Cerebral Ischemia-reperfusion Injury |
| XIAO Xu, SHI Wenda, CAI Chunhua, et al |
| The Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the effects of Matrine on Th1/Th2 subsets and activated cytokines in peripheral blood of rats with cerebral ischemia reperfusion injury. Methods: 50 SD rats were randomly divided into 5 groups, namely sham operation group, model group, matrine pretreatment group (4mg/kg, 8mg/kg, 16 mg/kg). 48 hours before operation, rats in both sham operation group and model group were gave saline by intraperitoneal injection, other drug pretreatment group were gaven matrine injection, respectively, bid for 2 days. Cerebral ischemia-reperfusion injury model in rats were established. 4h, 8h, 16h, 24h later, T cell subsets in femoral artery blood were detected by flow cytometry. Then the serum was separated and the cytokines (IL-1β, TNF-α, IL-4,and IL-10) were detected by ELISA. Results: The matrine pretreatment can significantly reduce the level of CD4+T cells in peripheral blood (P<0.05 or P<0.01), increase the level of CD8+T cells (P<0.05 or P<0.01), reduce the level of proinflammatory cytokines TNF-α and IL-1β in the activated Th1 cell (P<0.05 or P<0.01). Increase the level of anti-inflammatory cytokines IL-4 and IL-10 in the activated Th2 cells (P<0.05 or P<0.01). Conclusion: Matrine can play a neuroprotective role on cerebral ischemia reperfusion injury in rats by regulating Th1/Th2 cell subsets, adjusting cytokines in activatedTh1/Th2 cell and inhibiting inflammation reaction.
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2018, Vol. 24 
