旋毛虫肌幼虫虫体蛋白诱导小细胞肺癌H446细胞凋亡及C-kit表达研究

doi:10.3969/j.issn.1006-6233.2018.08.001

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Abstract Objective: To investigate the effect of apoptosis of somatic proteins from Trichinella spiralis muscle larva on small cell lung cancer H446 cells and correlation of C-kit expression. Methods: The H446 cells were divided into groups, somatic proteins of Trichinella spiralis muscle larva as experiment group, cis-platinum(DDP) as positive control group and untreated cells as negative control group. The effects of somatic protein and DDP on the proliferation activity of H446 cells were detected by MTT colorimetry; the cell apoptosis rate of H446 cells were induced by somatic proteins and DDP by flow cytometry(FCM) respectively; the transcriptional level of C-kit mRNA and protein expression of C-kit protein were detected by Real time PCR and Western Blot method respectively. Results: The results of MTT detection showed that the proliferation of H446 cells decreased with the increase of the concentration of insect body protein and the prolongation of the time of H446 cells, and the insect body protein had a significant inhibitory effect on H446 cells, showing a dose time effect. Flow cytometry showed that somatic proteins and DDP could induce apoptosis of H446 cells. Real time PCR and Western Blot showed that the transcriptional level of C-kit mRNA and protein expression of C-kit protein were decreased of somatic proteins group and DDP group respectively, compared with negative control group. Conclusion: The somatic protein of Trichinella spiralis muscle larvae can inhibit the growth of H446 cells and induce apoptosis of H446 cells. C-kit gene may be one of the molecular targets for the treatment of small cell lung cancer.

Key words： Trichinella spiralis muscle larva Somatic proteins Cell apoptosis C-kit

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2018.08.001 OR http://www.hbyxzzs.cn/EN/Y2018/V24/I8/1233

[1] Yu S, Yang C S, Li J, et al. Cancer prevention research in China [J]. Cancer Prevention Research, 2015, 8 (8): 662~674.
[2] 田甜,张波,刘洋,等.小细胞肺癌治疗现状[J].中国药物与临床,2014,14(11):1521~1523.
[3] 段玲欣,关学敏,张传生,等.旋毛虫抗肿瘤机制研究进展[J].中国寄生虫学与寄生虫病杂志,2011,29(2):142~146.
[4] Yu Y R, Deng M J, Lu W W, et al. Endoplasmic reticulum stress-mediated apoptosis is activated in intestines of mice with Trichinella spiralis infection.[J]. Experimental Parasitology, 2014, 145(1):1~6.
[5] Luo J M, Yu L, Xie G C, et al. Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs [J]. Parasitology, 2017, 144(1): 793~800.
[6] Wang X L, Liu M Y, Sun S M, et al. An anti-tumor protein produced by Trichinella spiralis induces apoptosis in human hepatoma H7402 cells [J]. Veterinary Parasitology, 2013, 194(2-4): 186~188.
[7] 常红敏,赵蕾,王小杰,等.旋毛虫排泄分泌蛋白对小细胞肺癌NCI-H446细胞凋亡的影响[J].中国寄生虫学与寄生虫病杂志,2014,32(4):299~303.
[8] 罗婧梅,程露阳,关晓东,等.质谱法分析旋毛虫肌幼虫排泄分泌蛋白的组分[J].中国寄生虫学与寄生虫病杂志,2016,34(1):53~57.
[9] 刘菁,刘晓雷,白雪,等.旋毛虫A200711蛋白对裸鼠H7402细胞实体瘤的抑制作用[J].中国兽医学报,2015,35(9):1483~1487.
[10] 段玲欣,关学敏,张传生,等.旋毛虫抗肿瘤机制研究进展[J].中国寄生虫学与寄生虫病杂志,2011,29(2):142~146.
[11] 夏钰弘,杨树东,郭庆,等.胸腺瘤组织C-kit、PTEN表达变化及其与WHO分型、患者预后的关系[J].山东医药,2018,58(4):86~88.
[12] Lebron M B, Brennan L, Damoci C B, et al. A human monoclonal antibody targeting the stem cell factor receptor (c-Kit) blocks tumor cell signaling and inhibits tumor growth.[J]. Cancer Biology & Therapy, 2014, 15(9):1208~1218.