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| Application of Immunofluorescence and other Special Staining in Pathological Diagnosis of Renal Biopsy |
| LUO Jiaoxiu, CHU Bing, CAO Xiaoshan, et al |
| Zhongshan Affiliated Hospital, Sun Yat-sen University, Guangdong Zhongshan 528403, China |
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Abstract Objective: To analyze the effect of immunofluorescence and other special staining methods in the pathological diagnosis of renal biopsy. Methods: 58 patients with chronic kidney disease admitted in our hospital from September 2014 to September 2016 were included in the object of study. All patients underwent renal biopsy puncture to obtain tissue using paraffin, respectively by immunofluorescence, six staining of methanol method, Congo red staining and mucin (PAS) and three Masson dye staining, staining results in different ways. Results: The positive rates of IgA, IgM, IgG, C3 and Clq in the patients with different types of nephropathy were significantly higher than those of IgA nephropathy and membranous nephropathy in patients by immunofluorescence staining. In the comparison of different staining methods, it was found that the positive rate of IgA, IgM, IgG, C3 and Clq detected by immunofluorescence staining was significantly lower than that of other staining methods, P<0.05. There was no difference in the diagnostic rate of other staining methods, P>0.05. Conclusion: Comparing with other methods, the rate of pathological diagnosis of renal biopsy is lower than that of other methods. It is recommended to use other special staining methods to diagnose renal tissue.
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2017, Vol. 23 
