羊蹄根对免疫性血小板减少性紫癜模型小鼠转化生长因子-β<sub>1</sub>及其受体的影响

doi:10.3969/j.issn.1006-6233.2017.05.011

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Abstract Objective: To observe the effects of Rumex japonicus Houtt extract on transforming growth factor-β₁ and its receptors in thrombocytopenic purpura mice model. Methods: 40 BALB/C mice were randomly divided into positive control group, model group, rumex japonicus houtt extract group and negative control group. All ITP mouse model were made by injected guinea pig-antimouse platelet serum (GP-APS) except negative control group. The expressions of TGF-β₁ and their receptors TGF-βR in the peripheral blood of mice were detected by the real-time PCR and Western Blot, and differences of their expression levels were analyzed. Results: The expression of TGF-β₁ in ITP model group was significantly higher than that in the negative control group (P<0.05), while the TGF-β mRNA and TGF-β protein expression in ITP model group were significantly lower than that in the negative control group (P<0.05). The expression of TGF-β₁ in rumex japonicus houtt extract group was significantly lower than that in the ITP model group (P<0.05), while the TGF-β mRNA and TGF-β protein expression in rumex japonicus houtt extract group were significantly higher than that in the ITP model group (P<0.05). Conclusion: Rumex japonicus houtt extract can regulate the immune function of ITP by in fluencing the expression of transforming growth factor-β₁ and its receptors.

Key words： Rumex japonicus houtt extract Immune thrombocytopenic purpura Model mouse Transforming growth factor-β₁ Receptors

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	GAO Yang
	JIANG Zujun

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GAO Yang,JIANG Zujun. Effects of Rumex Japonicus Houttextract on Transforming Growth Factor-β₁ and Receptors in Thrombocytopenic Purpura Mice[J]. 河北医学, 2017, 23(5): 741-744.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2017.05.011 OR http://www.hbyxzzs.cn/EN/Y2017/V23/I5/741

[1] Cooper N,Bussel J.The pathogenesis of immune thrombocytopaenic purpura[J].Br Haematol,2006,133(4):364~374
[2] Shan NN,Ji XB,Wang X,et al.In vitro recovery of Th1/Th2 balance in PBMCs from patients with immune thrombocytopenia through the actions of IL-18BPa/Fc[J].Thromb Res,2011,128(6):119~24.
[3] Wang J D,Chang T K,Lin H K,et al.Reduced expression of transforming growth factor-β₁ and correlated elevation of interleukin-17 and interferon-γ in pediatric patients with chronic primary immune thrombocytopenia(ITP)[J].Pediatr Blood Cancer,2011,57(4):636~640.
[4] Zver S,Zupan IP,Cernelc P.Cyclosporin A as an immunosuppressive treatment motality for patients with refractory autoimmune thrombocytopenic purpura after splenectomy failure[J].Int Hematol,2006;83(3):238-242.
[5] 马健康,姜艳霞,马洪波,等.羊蹄根提取物提取物对血小板减少症模型小鼠造血系统的作用[J].吉林大学学报,2009,35(1):82~86.
[6] 杨宇飞,周霭祥,麻柔.免疫性血小板减少性紫癜动物模型的建立[J].中华血液学杂志,1994,15(3):160~161.
[7] 中华医学会血液学分会血栓与止血学组.成人原发免疫性血小板减少症诊治的中国专家共识(修订版)[J].中华血液学杂志,2011,32(3.):214~216.
[8] Sun T,Zhang D,Yang Y,et al.Interleukin 35 may contribute to the loss of immunological self-tolerance in patients with primary immune thrombocytopenia[J].Br Haematol,2015,169(2):278~285.
[9] Xiao J,Zhang C,Zhang X,et al.Transplantation of adipose-derived mesenchymal stem cells into a murine model of passive chronic immune thrombocytopenia[J].Transfusion,2012,52(12):2551~2558.
[10] Lu L,Wang LS,Cooper RJ,et al.Suppressive effects of TNF-alpha,TGF-beta 1,and chemokines on megakaryocytic colony formation in CD+34 cells derived from umbilical cord blood compared with mobilized peripheral blood and bone marrow[J].Hematother Stem Cell Res,2000,9(2):195.
[11] 马金华,王晖.羊蹄根提取物汤与西药合用治疗原发性血小板减少性紫癜28例[J].中国药师,2003,6(5):292~293
[12] Hartel JV,Granchelli JA,Hudecki MS,et al.Impact of prednisone on TGF-beta1 and collagen in diaphragm muscle from mdx mice[J].Muscle Nerve,2001,24(3):428~432.