|
|
|
| Study on the Effect and Mechanism of Injection of Raltitrexed on |Cancer Cells in Gastric Cancer Patients |
| MA Shihua, SHI Nengsheng, et al |
| The People's Hosptal in Lishui District of Nanjing, Jiangsu Nanjing 211200, China |
|
|
|
|
Abstract Objective: To investigate the effect and mechanism of injection of raltitrexed on cancer cells in gastric cancer patients. Methods: 84 nude mice were purchased to establish the human gastric cancer cell MGC-803 subcutaneous tumormodel,after successful modeling ,micewere randomly divided into 3 groups, the conventional group received intraperitoneal injection of saline, the raltitrexed group were given intraperitoneal injection of raltitrexed, the 5-Fu group were given intraperitoneal injection of 5-Fu, nude miceweight, tumor volume, tumorweight ,inhibition rate,apoptosis rate, cycle distribution of cancer cellsafter intervention were observed. Results: Nude miceweight, tumor volume ,tumor weight of the conventional groupwere significantly higher than those of the raltitrexed group and the 5-Fu group, the difference had statistical significance (P<0.05);The raltitrexed group'snude mice weight was higher than that of the 5-Fu group, the difference had no statistical significance (P>0.05), tumor volume, tumor weight of the raltitrexed group were lower than those of the 5-Fu group, the difference had statistical significance(P<0.05);The nude mice cancer cells S phase distribution of the conventional group was significantly higher than that of the raltitrexed group and the 5-Fu group, the difference had statistical significance(P<0.05); The S phase distribution of the raltitrexed group and the 5-Fu group had no significant difference, the difference hadno statistical significance (P>0.05);There was no significant difference between the raltitrexedgroup and the 5-Fu group in G0/G1 , the difference hadno statistical significance (P>0.05);G0/G1 phase distribution of the raltitrexed group and the 5-Fu group was lower than that of the conventional group, the difference had statistical significance (P<0.05); G2/M phrase distribution among the three groups has no obvious difference, the difference hadno statistical significance(P>0.05);The conventional group's cancer cell inhibition rate, apoptosis rate were significantly lower than those of the raltitrexed group and the 5-Fu group, the difference had statistical significance(P<0.05); While those of the raltitrexed group were significantly higher than those of the 5-Fu group, the difference had statistical significance (P<0.05). Conclusion: Adoptingraltitrexed for gastric cancer patients has similaranti-tumor effect with 5-Fu, which can effectively inhibit the growth of human gastric cancer cell MGC-803 xenograft tumor in nude mice and induce apoptosis of cancer cells.
|
|
|
|
|
|
[1]封革,陈慧敏,胡萍,等.雷替曲塞对微卫星高度不稳定的结肠癌细胞凋亡的作用及临床意义[J].中国临床研究,2016,29(12):1610~1613.
[2] 张霞,陈迎平,祁峰.华蟾素注射液对人肝癌肿瘤细胞HepG2增殖抑制作用观察及其作用机制研究[J].湖北中医药大学学报,2016,18(6):25~28.
[3] 徐瀚峰,朱传东,王礼学,等.奥曲肽联合雷替曲塞对胃癌细胞株SGC-7901增殖的影响[J].现代中西医结合杂志,2013,22(30):3317~3319.
[4] 赵焱,彭翔,黄风云.MicroRNA-93对胶质瘤癌细胞侵袭和转移的影响及其作用机制研究[J].中国现代医学杂志,2017,27(1):64~70.
[5] 邢蓉,屈彪,宋佳伟,等.黄芪注射液多剂量给药对雷替曲塞在大鼠体内药动学行为影响[J].中国中药杂志,2014,39(11):2140.
[6] 李如标,张为民,高英.注射用雷替曲塞在晚期实体瘤患者体内的药动学[J].中国新药与临床杂志,2011,30(9):709~712.
[7] 鲁赛红,夏韬.伊立替康联合替吉奥、雷替曲塞或氟尿嘧啶治疗进展期胃癌的临床观察[J].中国药房,2017,28(11):1548~1551.
[8] 阎昭,薛津怀,朱仲玲.注射用雷替曲塞在结、直肠肿瘤患者体内的药动学[J].中国医院药学杂志,2010,30(9):749~752.
[9] 侯计平,韩恩崑,杜庆云.雷替曲塞联合吉西他滨治疗原发性肝癌的临床研究[J].现代药物与临床,2017,32(2):263~266.
[10] 邢蓉,周恺,宋佳伟.黄芪注射液单次给药对雷替曲塞在大鼠体内药动学研究[J].中国中药杂志,2013,38(11):1823~1826.
[11] 陈艳,朱为民,周冬霞,等.斑蝥酸钠维生素B6联合雷替曲塞和奥沙利铂治疗晚期结直肠癌的临床研究[J].现代药物与临床,2016,31(11):1784~1787.
[12] 朱美琴,夏俊贤,徐敏,等.雷替曲塞与5-氟尿嘧啶治疗老年晚期结直肠癌临床疗效对比分析[J].海南医学,2014,25(6):805~807.
[13] 宁昌,王洋,王志刚.雷替曲塞治疗老年晚期结直肠癌21例临床观察[J].海南医学,2011,22(10):61~63. |
|
|
|
2017, Vol. 23 
