LncRNA H19靶向调控miR-19b-3p/SOX9通路对脂肪间充质干细胞向成骨细胞分化的影响

doi:10.3969/j.issn.1006-6233.2024.07.04

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Abstract Objective: To investigate the effects of LncRNA H19 targeting miR-19b-3p/SOX9 pathway on the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs). Methods: Human ADMSCs were cultured and identified in vitro. The cells were divided into five groups: control group (regular culture), H19 group (transfected with LncRNA H19 lentivirus overexpression vector), LncRNA-NC group (transfected with lentivirus empty vector), miR-19b-3p group (co-transfected with LncRNA H19 lentivirus overexpression vector and miR-19b-3p mimic lentivirus vector), and miR-NC group (co-transfected with LncRNA H19 lentivirus overexpression vector and miR-NC lentivirus vector). The expression of LncRNA H19, miR-19b-3p, SOX9, zinc finger osteoblast-specific transcription factor (Osx), osteocalcin (OCN), and RUNX2 was detected in each group. ALP staining and Alizarin Red staining were observed in each group, cell proliferation activity and ALP activity were compared, and the expression of SOX9, BMP-2, and OPN proteins in each group was analyzed to verify the targeted relationship between LncRNA H19 and miR-19b-3p. Results: Compared with the control group and LncRNA-NC group, the expression of LncRNA H19 and SOX9, Osx, OCN, and RUNX2 mRNA, as well as the expression of SOX9, BMP-2, and OPN proteins, ALP activity, and the amount of mineralized nodule formation were increased in the H19 group, while the expression of miR-19b-3p was decreased (P<0.05). Compared with the H19 group and miR-NC group, the expression of LncRNA H19 and SOX9, Osx, OCN, and RUNX2 mRNA, as well as the expression of SOX9, BMP-2, and OPN proteins, ALP activity, and the amount of mineralized nodule formation were decreased in the miR-19b-3p group, while the expression of miR-19b-3p was increased (P<0.05). There was no statistical difference in the absorbance of each group at 24h, 48h, and 72h (P>0.05). There was a targeted relationship between LncRNA H19 and miR-19b-3p. Conclusion: LncRNA H19 can negatively regulate miR-19b-3p, upregulate SOX9, and contribute to the differentiation of human ADMSC cells into osteoblasts. LncRNA H19 can negatively regulate miR-19b-3p, upregulate SOX9, and promote the osteogenic differentiation of human ADMSCs.

Key words： Long non coding ribonucleic acid H19 MiR-19b-3p/SRY-box transcription factor 9 pathway Adipose derived mesenchymal stem cells Osteoblast differentiation

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	FENG Lu
	WANG Yao
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FENG Lu,WANG Yao,et al. LncRNA H19 Targets miR-19b-3p/SOX9 Pathway to Promote Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells[J]. HeBei Med, 2024, 30(7): 1075-1081.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.07.04 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I7/1075

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