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| Effect of Combined Ropivacaine and Sufentanil Anesthesia in Mid-Pregnancy on Progeny Brain-Derived Neurotrophic Factor Expression and Cognitive Function in SD Rats |
| DI Yan, LI Lihan, JIAO Shanshan, et al |
| No.980th Hospital of Joint Logistic Support Force, People's Liberation Army, Hebei Shijiazhuang 050082, China |
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Abstract Objective: To investigate the effects of combined ropivacaine and sufentanil anesthesia in mid-pregnancy on progeny brain-derived neurotrophic factor (BDNF) expression and cognitive function in SD rats. Methods: The study hypothesized that maternal treadmill exercise during pregnancy could prevent neurotoxicity induced by ropivacaine and sufentanil. In the first experiment, pregnant rats were exposed to a 100 μL mixture of sufentanil (0.25 μg/μL) and ropivacaine (0.125 μg/μL) (R&G) on gestational (G) day 14, or were exposed continuously on G13, G14, and G15 (R&Gx3). In the second experiment, pregnant rats in the exercise group were forced to run on a treadmill for 60 minutes daily throughout pregnancy. The TrkB antagonist ANA-12 was used to investigate whether the BDNF/TrkB signaling pathway was involved in the neuroprotection provided by maternal exercise. Results: Repeated but not single exposure to ropivacaine and sufentanil led to a decrease in histone acetylation and BDNF expression in fetal brain tissue and postnatal hippocampus. Spatial learning and memory impairments were compromised, accompanied by a reduction in dendritic spine number. Maternal exercise mitigated these effects, but the TrkB antagonist ANA-12 abolished the beneficial effects of maternal exercise. Conclusion: Multiple (but not single) exposures to ropivacaine and sufentanil in mid-pregnancy in pregnant rats lead to long-term learning and memory impairments in offspring. Maternal exercise improves postnatal neurocognitive deficits by enhancing histone acetylation and activating downstream BDNF/TrkB signaling.
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2024, Vol. 30 
