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| Involvement of miRNA-93-5p in Cervical Cancer Progression by Regulating FBXW7 Gene |
| WANG Jiang |
| Nanchong Central Hospital / Affiliated to North Sichuan Medical College, Sichuan Nanchong 637000, China |
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Abstract Objective: To explore whether miRNA-93-5p can affect the progression of cervical cancer by regulating FBXW7 gene. Methods: Twenty clinical cervical cancer patient tissues were collected and divided into cancer-adjacent tissues and cervical cancer tissues. RT-qPCR was used to detect the levels of miRNA-93-5p and FBXW7 mRNA in cervical tissues. The miRNA-93-5p and FBXW7 mRNA levels were also measured in normal cervical epithelial cells (H8) and cervical cancer cells (Caski, HeLa, C33A). The cervical cancer cell line C33A was selected for further experiments. C33A cells were divided into the control group (normal culture), miRNA-93-5p inhibition group (transfected with low-expression miRNA-93-5p), and FBXW7 activation group (transfected with high-expression FBXW7). After 48 hours of transfection, cells were collected, and RT-qPCR was used to detect miRNA-93-5p and FBXW7 mRNA levels. Western blotting measured FBXW7 protein levels in C33A cells. Flow cytometry assessed apoptosis rates, plate colony assays examined cloning ability, scratch tests evaluated migration capacity, and Transwell assays measured invasion ability. Results: In comparison with cancer-adjacent tissues, cervical cancer tissues showed increased miRNA-93-5p mRNA levels and decreased FBXW7 mRNA levels (P<0.001). Compared with H8 cells, Caski, HeLa, and C33A cells exhibited increased miRNA-93-5p mRNA levels and decreased FBXW7 mRNA levels (P<0.05). Subsequent experiments were conducted using the C33A cervical cancer cell line. In the miRNA-93-5p inhibition group, miRNA-93-5p mRNA levels significantly decreased (P<0.001), apoptosis rates increased (P<0.001), cloning ability decreased (P<0.01), migration distance reduced (P<0.01), invasion ability decreased (P<0.01), and FBXW7 protein and mRNA levels significantly increased (P<0.01) compared with the control group. In the FBXW7 activation group, FBXW7 mRNA levels significantly increased (P<0.01), apoptosis rates increased (P<0.001), cloning ability decreased (P<0.001), migration distance reduced (P<0.01), invasion ability decreased (P<0.01) compared with the control group. Conclusion: miRNA-93-5p can affect the apoptosis, proliferation, migration and invasion of cervical cancer cells by regulating FBXW7 gene.
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[1] Shrestha AD,Neupane D,Vedsted P,et al.Cervical cancer prevalence,incidence and mortality in low and middle income countries:a systematic review[J].Asian Pac Cancer Prev,2018,19(2):319-324.
[2] Hill M,Tran N.miRNA interplay:mechanisms and consequences in cancer[J].Dis Model Mech,2021,14(4):47662.
[3] Menon A,Abd-Aziz N,Khalid K,et al.miRNA:a promising therapeutic target in cancer[J].Int Mol Sci,2022,23(19):11502.
[4] Shen E,Wang X,Liu X,et al.MicroRNA-93-5p promotes epithelial-mesenchymal transition in gastric cancer by repressing tumor suppressor AHNAK expression[J].Cancer Cell Int,2020,12(20):76.
[5] Wang X,Liao Z,Bai Z,et al.MiR-93-5p promotes cell proliferation through down-regulating PPARGC1A in hepatocellular carcinoma cells by bioinformatics analysis and experimental verification[J].Genes (Basel),2018,9(1):51.
[6] Sun XY,Han XM,Zhao XL,et al.MiR-93-5p promotes cervical cancer progression by targeting THBS2/MMPS signal pathway[J].Eur Rev Med Pharmacol Sci,2019,23(12):5113-5121.
[7] Fan J,Bellon M,Ju M,et al.Clinical significance of FBXW7 loss of function in human cancers[J].Mol Cancer,2022,21(1):87.
[8] Yeh CH,Bellon M,Nicot C.FBXW7:a critical tumor suppressor of human cancers[J].Mol Cancer,2018,17(1):115.
[9] Ben W,Zhang G,Huang Y,et al.MiR-27a-3p regulated the aggressive phenotypes of cervical cancer by targeting FBXW7[J].Cancer Manag Res,2020,29(12):2925-2935.
[10] Small W Jr,Bacon MA,Bajaj A,et al.Cervical cancer:a global health crisis[J].Cancer,2017,123(13):2404-2412.
[11] Hu Z,Ma D.The precision prevention and therapy of HPV-related cervical cancer:new concepts and clinical implications[J].Cancer Med,2018,7(10):5217-5236.
[12] Bhatla N,Aoki D,Sharma DN,et al.Cancer of the cervix uteri:2021 update[J].Int Gynaecol Obstet,2021,Suppl 1(Suppl 1):28-44. |
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2024, Vol. 30 
