LncRNA PINK1-AS调节miR-134-5p/SERPINE1轴对胃癌细胞增殖凋亡和上皮间质转化的影响

doi:10.3969/j.issn.1006-6233.2023.12.013

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Abstract Objective: To investigate the effects of LncRNA PINK1-AS on the proliferation, apoptosis and epithelial mesenchymal transformation (EMT) of gastric cancer cells by regulating the miR-134-5p/serine protease inhibitor clade E member 1 (SERPINE1) axis. Methods: QRT-PCR was applied to detect the expression levels of PINK1-AS, miR-134-5p, and SERPINE mRNA in 60 cases of gastric cancer tissues and 60 cases of adjacent tissues. Gastric cancer cells MKN28 were cultured in vitro and grouped into control group, si-NC group, si-PINK1-AS group, pc-NC group, pc-PINK1-AS group, miR-NC group, and miR-134-5p mimics group; QRT-PCR was applied to detect the expression levels of PINK1-AS, miR-134-5p, and SERPINE mRNA in cells of each transfection group, Cck-8 was applied to detect cell proliferation; Flow cytometry was applied to detect cell apoptosis rate; Transwell test was applied to detect cell migration and invasion; Western blot was applied to detect the expression levels of SERPINE1, Ki-67, cleaved caspase-3, E-cadherin, N-cadherin, and vimentin proteins in cells. Double Luciferase reporter gene experiment was applied to detect the targeting relationship between PINK1-AS and miR-134-5p, and between miR-134-5p and LncRNA SERPINE1. Results: The expression of PINK1-AS and SERPINE mRNA in gastric cancer tissue was obviously higher than that in adjacent tissues, while the expression of miR-134-5p was lower than that in adjacent tissues (P<0.05). Down-regulation the expression of PINK1-AS can reduce the PINK1-AS, SERPINE mRNA, OD450 values (24, 48h), cell migration and invasion, the expression of SERPINE1, Ki-67, N-cadherin, vimentin proteins in MKN28 cells ,increased the expression of miR-134-5p, cell apoptosis rate, the expression of cleaved caspase-3, and E-cadherin proteins (P<0.05). Overexpression of PINK1-AS can increase the PINK1-AS, SERPINE mRNA, OD450 values (24, 48h), cell migration and invasion, the expression of SERPINE1, Ki-67, N-cadherin, vimentin proteins in MKN28 cells, the expression of miR-134-5p, cell apoptosis rate, the expression of cleaved caspase-3, and E-cadherin proteins obviously decreased (P<0.05). Overexpression of miR-134-5p increased miR-134-5p expression, apoptosis rate, cleaved caspase-3, and E-cadherin protein expression, and decreased SERPINE mRNA, OD450 value (24, 48h), cell migration and invasion number, SERPINE1, Ki-67, N -cadherin, and vimentin protein expression (P<0.05). Conclusion: LncRNA PINK1-AS can regulate the miR-134-5p/SERPINE1 axis, thereby affecting proliferation, apoptosis and EMT of gastric cancer cells.

Key words： Gastric cancer cells LncRNA PINK1-AS miR-134-5p/SERPINE1 axis Epithelial mesenchymal transformation

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	LI Fan
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LI Fan,SONG Yutao,WANG Feng, et al. Effects of LncRNA PINK1-AS on the Proliferation Apoptosis and Epithelial Mesenchymal Transformation of Gastric Cancer Cells by Regulating the miR-134-5p/SERPINE1 Axis[J]. HeBei Med, 2023, 29(12): 2004-2011.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.12.013 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I12/2004

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