EGFR/ALK野生型晚期NSCLC患者血清miR-499 miR-144-3p变化及对预后的预测价值

doi:10.3969/j.issn.1006-6233.2023.11.011

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Abstract Objective: To analyze the variations in serum microRNA (miR-499) and miR-144-3p among patients with advanced non-small cell lung cancer (NSCLC) of epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) wild-type,and evaluate their predictive value for prognosis. Methods: Clinical data were collected from 107 patients with EGFR/ALK wild-type advanced NSCLC who were admitted to the hospital between January 2018 and 2020 (NSCLC group).Additionally,107 healthy subjects who underwent physical examinations during the same period were selected as the control group.Real-time fluorescence RCR technology was employed to measure the expression of serum miR-499 and miR-144-3p.Differences between the two groups were compared,and changes in serum miR-499 and miR-144-3p expressions were assessed across patients with advanced NSCLC exhibiting various clinicopathological characteristics.Follow-up began at discharge and ended in January 2023,considering death as the endpoint event.The prognostic survival rate was compared between advanced NSCLC patients with high and low expressions of miR-499 and miR-144-3p,using an expression level greater than the average value as the criterion. Results: In the NSCLC group,relative expression levels of serum miR-499 [(0.74±0.18) vs (1.05±0.20)] and miR-144-3p [(0.82±0.19) vs (1.22±0.21)] were significantly lower compared to the control group (P<0.05).Among patients with advanced NSCLC,the relative expression levels of serum miR-499 [(0.64±0.17) vs (0.86±0.21)] and miR-144-3p [(0.71±0.16) vs (0.95±0.22)] were lower in stage IV patients compared to stage ⅢB and ⅢC patients (P<0.05).Furthermore,patients with lower differentiation exhibited lower expression levels compared to those with moderate-to-high differentiation [(0.63±0.16) vs (0.85±0.21),(0.70±0.15) vs (0.94±0.22),P<0.05].The follow-up duration for advanced NSCLC patients ranged from 4 to 56 months,with a median follow-up period of 34 months.Patients with high serum miR-499 expression had a significantly higher survival rate compared to those with low expression (P<0.05),as did patients with high serum miR-144-3p expression (P<0.05). Conclusion: Serum miR-499 and miR-144-3p exhibit low expression in patients with EGFR/ALK wild-type advanced NSCLC.Furthermore,their expression levels are even lower in stage IV patients and those with lower differentiation.Importantly,these expression levels hold predictive value for prognostic survival rates.

Key words： Non-small cell lung cancer Advanced EGFR/ALK wild-type miR-499 miR-144-3p Prognosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.11.011 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I11/1816

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