非小细胞肺癌矮小同源盒基因2和Ras相关结构域蛋白家族1A基因甲基化的意义

doi:10.3969/j.issn.1006-6233.2023.10.005

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Abstract Objective: To explore the relationship between the gene methylation of dwarf stature homeobox-containing gene (SHOX2) and Ras-related domain protein family 1A (RASSF1A) and the invasion and metastasis of non-small cell lung cancer (NSCLC). Methods: Methylation-specific PCR (MSP) was used to analyze the methylation status of SHOX2 and RASSF1A gene promoter regions in NSCLC and its corresponding adjacent tissues, and the relationship between methylation status and clinical pathological features of patients was analyzed. Results: The methylation rates of SHOX2 and RASSF1A gene promoter regions in NSCLC cancer tissues were 68.97% (80/116) and 33.62% (39/116) respectively, which were significantly higher than those in normal adjacent tissues with 12.93% (15/116) and 10.34% (12/116) (P<0.05). SHOX2 and RASSF1A gene methylation distribution had no relationship with gender, age, smoking history, tumor diameter, and differentiation degree of NSCLC patients (P>0.05), but had something to do with TNM stage, pathological classification, lymph node metastasis, and survival time (P<0.05). The methylation rate of SHOX2 gene in patients with TNM stage IIIa +IIIb was significantly higher than that in patients with stage I + II (P<0.05), and the gene methylation rate of patients with squamous carcinoma was significantly higher than that of patients with adenocarcinoma (P<0.05), and the gene methylation rate in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis (P<0.05), and the gene methylation rate in patients with survival time <2 years was significantly higher than that in patients with survival time ≥ 2 years (P<0.05). The RASSF1A gene methylation rate in patients with TNM stage IIIa + IIIb was significantly higher than that in patients with stage I + II (P<0.05), and the gene methylation rate of patients with adenocarcinoma was significantly higher than that in patients with squamous carcinoma (P<0.05), and the gene methylation rate in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis (P<0.05), and the gene methylation rate in patients with survival time< 2 years was significantly higher than that in patients with survival time≥ 2 years (P<0.05). Conclusion: The methylation of SHOX2 and RASSF1A gene promoter regions in NSCLC tissues may be one of the reasons for the invasion and metastasis of NSCLC. Detection of SHOX2 and RASSF1A gene promoter methylation can provide reference value for diagnosis of NSCLC and evaluation of prognosis.

Key words： Dwarf stature homeobox-containing gene Ras-related domain protein family 1A Non-small cell lung cancer Methylation

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	Articles by authors
	ZHANG Xuguang
	DA Haili
	XING Wenting

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ZHANG Xuguang,DA Haili,XING Wenting. Significance of Methylation of Dwarf Homology Box Gene 2 and Ras-Related Structural Domain Protein Family 1A Genes in Non-Small Cell Lung Cancer[J]. HeBei Med, 2023, 29(10): 1612-1617.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.10.005 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I10/1612

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