LncRNA LINC01194在非小细胞肺癌中对miR-302e的影响及机制研究

doi:10.3969/j.issn.1006-6233.2023.10.003

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Abstract Objective: To explore the impacts and mechanism of long non-coding RNA (lncRNA LINC01194) on miR-302e in non-small cell lung cancer (NSCLC). Methods: Real-time fluorescence quantitative PCR (qRT-PCR) was applied to detect the expression of lncRNA LINC01194 and miR-302e in NSCLC tissue, paracancerous tissue, human lung normal epithelial cells BEAS-2B, and NSCLC cell lines, respectively, to screen the best intervention cell line; NCI-H1975 cells were divided into: control group (normal culture), si-NC group (transfected with si-NC), si-LINC01194 group (transfected with si-LINC01194), si-LINC01194+anti miR-NC group (co-transfected with si-LINC01194 and anti miR-NC), and si-LINC01194+anti miR-302e group (co-transfected with si-LINC01194 and anti miR-302e); qRT-PCR was applied to detect the expression of lncRNA LINC01194 and miR-302e in cells; MTT assay, plate cloning assay, Transwell chamber flow cytometry were applied to detect cell proliferation, migration, invasion and apoptosis, respectively; Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-2 (MMP-2), B-cell lymphoma protein 2 (Bcl-2), and Bcl-2 associated X protein (Bax); and double Luciferase reporter gene experiment was applied to verify the targeting regulation relationship between lncRNA LINC01194 and miR-302e. Results: In NSCLC tissues and cells, the expression level of lncRNA LINC01194 increased, while the expression level of miR-302e decreased (P<0.05); inhibiting the expression of lncRNA LINC01194 was able to obviously inhibit the proliferation, migration, and invasion of NCI-H1975 cells, and promote cell apoptosis (P<0.05); inhibiting miR-302e expression was able to reverse the inhibitory effect of lncRNA LINC01194 expression on NCI-H1975 cell proliferation, migration, invasion, and promote cell apoptosis (P<0.05); double Luciferase reporter gene experiment confirmed that there was a targeted regulatory relationship between lncRNA LINC01194 and miR-302e. Conclusion: lncRNA LINC01194 is up-regulated and miR-302e is down-regulated in NSCLC tissues and cells, inhibiting lncRNA LINC01194 can up-regulate the expression of miR-302e, thereby inhibiting the proliferation, migration, and invasion of NSCLC cells and promoting their apoptosis.

Key words： lncRNA LINC01194 miR-302e Non small cell lung cancer

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.10.003 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I10/1600

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