黄芩苷调节Notch/JAG1信号通路对舌癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2023.09.06

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Abstract Objective: To investigate the impact of baicalin (Bai) on the malignant biological behavior of tongue cancer cells by regulating Notch/JAG1 signal pathway. Methods: Human oral epithelial cells HOEC was treated with 0, 10, 20, 30, 40, 50 and 100 μM Bai. Human tongue phosphorous carcinoma cell line CAL27 was grouped into CAL27 group (untreated CAL27 cells), L-Bai group (10μM Bai treated CAL27 cells), M-Bai group (20μM Bai treated CAL27 cells), H-Bai group (40μM Bai treated CAL27 cells), VPA group (8 mM Notch/JAG1 pathway activator VPA treated CAL27 cells), and H-Bai+VPA group (40μM Bai and 8 mM VPA co-treated CAL27 cells); MTT assay was applied to detect the effects of Bai on HOEC cytotoxicity and CAL27 cell viability; the apoptosis of CAL27 cells was detected by flow cytometry; Transwell was applied to detect the invasion and migration abilities of CAL27 cells; Western blot was applied to detect the expression of epithelial-mesenchymal transformation (EMT) protein and Notch/JAG1 signaling pathway protein. Results: 0~100μM Bai had no obvious toxic effect on HOEC cells. Compared with CAL27 group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in L-Bai group, M-Bai group and H-Bai group decreased obviously in turn (P<0.05), the apoptosis rate and the level of E-cadherin protein increased obviously in turn (P<0.05), in the VPA group, the above indicators had the opposite trends; compared with H-Bai group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in H-Bai+VPA group increased obviously (P<0.05), the apoptosis rate and the level of E-cadherin protein decreased obviously (P<0.05). Conclusion: Bai may affect the proliferation, apoptosis and EMT of tongue cancer cells by inhibiting Notch/JAG1 signaling pathway.

Key words： Baicalin Notch/JAG1 signal pathway Tongue cancer Proliferation Apoptosis Migration

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	WANG Huan
	XIA Li
	LI Wei
	et al

Cite this article:

WANG Huan,XIA Li,LI Wei, et al. Impact of Baicalin on Malignant Biological Behavior of Tongue Cancer Cells by Regulating Notch/JAG1 Signaling Pathway[J]. HeBei Med, 2023, 29(9): 1438-1443.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.09.06 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I9/1438

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