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| Impacts of Quercetin on the Biological Behavior of Thyroid Papillary Carcinoma Cells by Regulating SOCS3/STAT3 Signaling Pathway |
| HAN Ke, et al |
| The Fifth People's Hospital of Jinan, Shandong Jinan 250000, China |
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Abstract Objective: To investigate the impacts of quercetin (Que) on the biological behavior of thyroid papillary carcinoma (PTC) cells by regulating cytokine signal transduction inhibitor 3 (SOCS3)/signal transduction and activator of transcription 3 (STAT3) signaling pathway. Methods: TPC-1 cells were grouped into control group, Que low (Que L, 25 μmoL/L Que), medium (Que M, 50 μmoL/L Que), high (Que H, 100 μmoL/L Que) concentration groups, Que H+shRNA NC group (transfected with shRNA NC using Lipofectamine 2000 transfection kit, and then treated with 100 μmoL/L Que), Que H+SOCS3 shRNA group (transfected with SOCS3 shRNA using Lipofectamine 2000 transfection kit, and then treated with 100 μmoL/L Que), the apoptosis of TPC-1 cells was detected by flow cytometry; the changes in TPC-1 cell proliferation, migration, and invasion were detected by CCK-8 and Transwell experiments; the expression levels of SOCS3 and STAT3 mRNA in TPC-1 cells were detected by qRT-PCR; Western blot was applied to detect the expression levels of SOCS3, p-STAT3/STAT3, and phosphorylated non receptor tyrosine kinase Janus kinase 2 (p-JAK2)/JAK2 in TPC-1 cells. Results: Compared with the control group, the apoptosis rate of TPC-1 cells and the expression of SOCS3 mRNA and protein in Que-L, Que-M, and Que-H groups were obviously increased, the proliferation rate, the numbers of invasion and migration, the expression of STAT3 mRNA, p-STAT3/STAT3, and p-JAK2/JAK2 were obviously decreased (P<0.05); compared with Que-H+shRNA NC group, the apoptosis rate of TPC-1 cells and the expression of SOCS3 mRNA and protein in Que-H+SOCS3 shRNA group were obviously decreased, the proliferation rate, the numbers of invasion and migration, the expression of STAT3 mRNA, p-STAT3/STAT3, and p-JAK2/JAK2 were obviously increased (P<0.05). Conclusion: Que can inhibit the malignant biological behavior of PTC cell line TPC-1 by activating the SOCS3/STAT3 signaling pathway.
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2023, Vol. 29 
